Isolation of free-living dinitrogen-fixing bacteria and their activity in compost containing de-inking paper sludge

被引:33
作者
Beauchamp, CJ [1 ]
Lévesque, G
Prévost, D
Chalifour, FP
机构
[1] Univ Laval, Dept Phytol, Ste Foy, PQ G1K 7P4, Canada
[2] Agr & Agr Food Canada, Soil & Crops Res & Dev Ctr, Ste Foy, PQ G1V 2J3, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
dinitrogen-fixing bacteria; compost; paper sludge; glucose; Pseudomonas balearica; Pseudomonas putida;
D O I
10.1016/j.biortech.2005.04.041
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Knowledge of the microbiology of dinitrogen (N-2)-fixing bacteria in compost rich in de-inking paper sludge (DPS) is limited. Dinitrogen (N-2)-fixing bacteria from DPS composts were isolated and studied for their N-2-fixing activity in vitro and in vivo. Two Gram-negative N2-fixing isolates were identified as Pseudomonas. At 20 degrees C, both isolates revealed that N-2-fixing activity was higher than that of three arctic Pseudomonas strains. Their N-2-fixing activity was found to occur between 18 and 25 degrees C, a pattern that was similar to the reference isolate Azotobacter ATCC 7486. Composts successfully showed N-2-fixing activity after carbohydrate amendments both with and without inoculation of a N-2-fixing isolate. These results suggest that DPS composts support N-2-fixing bacteria and that N-2-fixing activity is dependent on a usable carbohydrate source. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1002 / 1011
页数:10
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