Microfluidic Synthesis of Cell-Type-Specific Artificial Extracellular Matrix Hydrogels

被引：78

作者：

Allazetta, Simone ^{[1
]}

Hausherr, Tanja C. ^{[1
]}

Lutolf, Matthias P. ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Inst Bioengn, Lab Stem Cell Bioengn, Lausanne, Switzerland

来源：

BIOMACROMOLECULES | 2013年 / 14卷 / 04期

关键词：

EMBRYONIC STEM-CELLS; DROPLET FORMATION; LADEN MICROGELS; EXPANSION; GENERATION; ENCAPSULATION; FABRICATION;

D O I：

10.1021/bm4000162

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Droplet microfluidic technology is applied for the high-throughput synthesis via Michael-type addition of reactive, micrometer-sized poly(ethylene glycol) (PEG) hydrogels ("microgels") with precisely controlled dimension and physicochemical properties. A versatile chemical scheme is used to modify the reactive PEG microgels with tethered biomolecules to tune their bioactive properties for the bioreactor culture and manipulation of various (stem) cell types.

引用

页码：1122 / 1131

页数：10

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