Protective Effect of Deer Bone Oil on Cartilage Destruction in Rats with Monosodium Iodoacetate (MIA)-Induced Osteoarthritis

被引:13
作者
Choi, Hyeon-Son [1 ]
Im, Suji [2 ]
Park, Je Won [3 ]
Suh, Hyung Joo [4 ]
机构
[1] Seoul Womens Univ, Dept Food Sci & Technol, Seoul 139774, South Korea
[2] DuPont Korea Inc, Seoul 135719, South Korea
[3] Korea Univ, Sch Biosyst & Biomed Sci, Seoul 136713, South Korea
[4] Korea Univ, Grad Sch, Dept Publ Hlth Sci, Seoul 136713, South Korea
关键词
osteoarthritis (OA); deer bone oil; chondrocyte; monosodium iodoacetate (MIA); micro-computed tomography (Micro-CT); ganglioside; MATRIX METALLOPROTEINASES; INFLAMMATORY RESPONSES; CHONDROCYTE APOPTOSIS; GANGLIOSIDES; EXTRACT; ANTLER; CYTOKINES; GLUTAMATE; PROTEIN; WEIGHT;
D O I
10.1248/bpb.b16-00565
中图分类号
R9 [药学];
学科分类号
100702 [药剂学];
摘要
The anti-osteoarthritic activity of the methanol fraction of deer bone oil extract (DBO-M) was evaluated in interleukin (IL)-1 beta-inflamed primary rabbit chondrocytes and in rats with monosodium iodoacetate (MIA)-induced osteoarthritis. The active compound in DBO-M was analyzed using a direct infusion liquid chromatography quadrupole (LCQ) ion-trap electrospray ionization (ESI)-mass spectrometer (MS). DBO-M significantly suppressed the IL-1 beta-induced sulfated-glycosaminoglycan (s-GAG) release from chondrocyte, and lowered mRNA levels of the collagen-degrading enzymes matrix metalloproteinase (MMP)-1 and MMP-3 in a dose-dependent manner. Upon treatment with high doses of DBO-M, the levels of IL-1 beta, tumor necrosis factor (TNF)-alpha, and IL-6 decreased by around 40, 70, and 50%, respectively, compared to the control in the serum of rats with MIA-induced osteoarthritis. Bone volume fraction (BV/TV) and trabecular thickness (Tb.Th) increased by over 40% in rats treated with DBO-M compared to the values reported for the MIA-treated control group, while trabecular separation (Tb.Sp) showed a significant decrease (ca. 38%), as confirmed through micro-computed tomography (CT) analysis of MIA-induced destruction of articular bones. Furthermore, direct infusion ESI-MS analysis showed that DBO-M contains gangliosides, which are glycosphingolipids with monosialic acid (GM3), as a major compound. Our results suggest that DBO-M effectively improves MIA-induced osteoarthritis by suppressing inflammatory responses, and that gangliosides could be one of the DSO-derived anti-inflammatory components.
引用
收藏
页码:2042 / 2051
页数:10
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