An integrated ''root tube'' bioreactor/separator for transformed root cultures

被引:3
作者
Green, KD [1 ]
Thomas, NH [1 ]
机构
[1] UNIV BIRMINGHAM, SCH CHEM ENGN, FLUID & SURFACE TRANSPORT TEAM, BIRMINGHAM B15 2TT, W MIDLANDS, ENGLAND
来源
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1996年 / 81卷 / 05期
关键词
transformed roots; bioreactor; Nicotiana; Datura; product release; product recovery; XAD-4; resin;
D O I
10.1016/0922-338X(96)85147-6
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The special morphology and biochemical characteristics of transformed hairy roots demand novel approaches in bioreactor concept to provide a favorable environment for root growth and product extraction. This paper describes our evaluation of the performance of an integrated bioreactor (1 l) and separation system. Growth rates of transformed Nicotiana glauca roots were comparable to those achieved in Erlenmeyer flasks, but the release of alkaloids was enhanced using production medium and heat treatment. Incorporation of an XAD-4 resin packed column separator also facilitated alkaloid release and improved recovery. In addition transformed roots of N. glauca, Nicotiana rustica and Datura stramonium were cultured in a 8 l bioreactor, achieving high growth rates and productivities comparable to reported values.
引用
收藏
页码:453 / 457
页数:5
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