Neutral amino acid transport in placental plasma membrane vesicles in the late pregnant rat - Evidence for a B-0-like transport system

被引:9
作者
Carbo, N [1 ]
LopezSoriano, FJ [1 ]
Argiles, JM [1 ]
机构
[1] UNIV BARCELONA,FAC BIOL,DEPT BIOQUIM & BIOL MOL,BARCELONA 08071,SPAIN
来源
EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY | 1997年 / 71卷 / 01期
关键词
amino acid; placenta; transport;
D O I
10.1016/S0301-2115(96)02614-0
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Rat placental plasma membrane vesicles have been used to study both alanine and leucine transport at late gestation. The results presented are consistent with the presence of more than two separate transport systems for neutral amino acids in the rat placenta. One system is clearly Na+-independent and transports alanine (K-M congruent to 2 mM; V-max congruent to 360 pmol ala/mg prot x 5 s), leucine (K-M congruent to 0.07 mM; V-max congruent to 100 pmol leu/mg prot x 5 s), serine, cysteine and 2-amino-2 norbornane carboxylic acid (BCH) showing similar properties to the L system, present in many cell types. The other systems are Na+-dependent and transport alanine (a, K-M congruent to 5 mM; V-max congruent to 3761 pmol ala/mg prot x 5 s; b, K-M congruent to 0.07 mM; V-max congruent to 376 pmol ala/mg prot x 5 s), cysteine, serine and leucine (K-M congruent to 0.2 mM; V-max congruent to 112 pmol leu/mg prot x 5 s) with different kinetic behaviour referring affinity and capacity. While one of them is sensitive to inhibition by methylaminoisobutyric acid (MeAIB), the other is a B-0-like system similar to that characterized in bovine brush-border enterocyte membrane vesicles. Copyright (C) 1997 Elsevier Science Ireland Ltd.
引用
收藏
页码:85 / 90
页数:6
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