In Situ Solid-State NMR Spectroscopy of Protein in Heterogeneous Membranes: The Baseplate Antenna Complex of Chlorobaculum tepidum

被引：21

作者：

Kulminskaya, Natalia V. ^{[1
,2
]}

Pedersen, Marie O. ^{[1
,2
,5
]}

Bjerring, Morten ^{[1
,2
]}

Underhaug, Jarl ^{[1
,2
,6
]}

Miller, Mette ^{[3
]}

Frigaard, Niels-Ulrik ^{[4
]}

Nielsen, Jakob T. ^{[1
,2
]}

Nielsen, Niels Chr. ^{[1
,2
]}

机构：

[1] Univ Aarhus, Ctr Insoluble Prot Struct InSPIN, Interdisciplinary Nanosci Ctr INANO, DK-8000 Aarhus C, Denmark

[2] Univ Aarhus, Dept Chem, DK-8000 Aarhus C, Denmark

[3] Univ So Denmark, Dept Biochem & Mol Biol, Odense, Denmark

[4] Univ Copenhagen, Dept Biol, DK-1168 Copenhagen, Denmark

[5] Novo Nordisk AS, Malov, Denmark

[6] Univ Bergen, Dept Biomed, N-5020 Bergen, Norway

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 28期

基金：

新加坡国家研究基金会;

关键词：

NMR spectroscopy; photosynthesis; proteins; solid-state structures; CHLOROBIUM-TEPIDUM; SECONDARY STRUCTURE; BACTERIOCHLOROPHYLL-C; CHLOROSOMES; ASSIGNMENT; MUTANT; CSMA; C-13;

D O I：

10.1002/anie.201201160

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A clever combination: An in situ solid-state NMR analysis of CsmA proteins in the heterogeneous environment of the photoreceptor of Chlorobaculum tepidum is reported. Using different combinations of 2D and 3D solid-state NMR spectra, 90 % of the CsmA resonances are assigned and provide on the basis of chemical shift data information about the structure and conformation of CsmA in the CsmA-bacteriochlorophyll a complex (see picture). Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：6891 / 6895

页数：5

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