High-performance liquid chromatographic determination of myocardial interstitial dihydroxyphenylglycol

被引：13

作者：

Takauchi, Y

Kitagawa, H

Kawada, T

Akiyama, T

Yamazaki, T

机构：

[1] NATL CARDIOVASC CTR,RES INST,DEPT CARDIAC PHYSIOL,SUITA,OSAKA 565,JAPAN

[2] NATL CARDIOVASC CTR,RES INST,DEPT CARDIOVASC DYNAM,SUITA,OSAKA 565,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY B | 1997年 / 693卷 / 01期

关键词：

dihydroxyphenylglycol;

D O I：

10.1016/S0378-4347(97)00003-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study describes a high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) for monitoring dihydroxyphenylglycol (DHPG) in the myocardial interstitial space. Using a cardiac dialysis technique, 10-mu l dialysates were sampled from the myocardial interstitial space (1-min fractions) and were injected directly into the HPLC-ED system. The in vitro quantification limit for DHPG was 250 fg in a 10-mu l injection. The basal DHPG concentration of dialysate was 181+/-46 pg/ml. This system offers a new possibility for monitoring myocardial interstitial DHPG levels.

引用

收藏

页码：218 / 221

页数：4

相关论文

共 10 条

[1] INVIVO MONITORING OF MYOCARDIAL INTERSTITIAL NOREPINEPHRINE BY DIALYSIS TECHNIQUE [J].

AKIYAMA, T ;

YAMAZAKI, T ;

NINOMIYA, I .

AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 261 (05) :H1643-H1647

[2] NEURONAL REUPTAKE OF NOREPINEPHRINE AND PRODUCTION OF DIHYDROXYPHENYLGLYCOL BY CARDIAC SYMPATHETIC-NERVES IN THE ANESTHETIZED DOG [J].

EISENHOFER, G ;

SMOLICH, JJ ;

COX, HS ;

ESLER, MD .

CIRCULATION, 1991, 84 (03) :1354-1363

[3] SOURCE AND PHYSIOLOGICAL SIGNIFICANCE OF PLASMA 3,4-DIHYDROXYPHENYLGLYCOL AND 3-METHOXY-4-HYDROXYPHENYLGLYCOL [J].

EISENHOFER, G ;

GOLDSTEIN, DS ;

ROPCHAK, TG ;

NGUYEN, HQ ;

KEISER, HR ;

KOPIN, IJ .

JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM, 1988, 24 (1-2) :1-14

[4] PLASMA DIHYDROXYPHENYLGLYCOL AND THE INTRANEURONAL DISPOSITION OF NOREPINEPHRINE IN HUMANS [J].

GOLDSTEIN, DS ;

EISENHOFER, G ;

STULL, R ;

FOLIO, CJ ;

KEISER, HR ;

KOPIN, IJ .

JOURNAL OF CLINICAL INVESTIGATION, 1988, 81 (01) :213-220

[5]

GRAEFE KH, 1981, N-S ARCH PHARMACOL, V318, P71

[6]

KOPIN IJ, 1985, PHARMACOL REV, V37, P333

[7] NEURONAL SODIUM HOMOEOSTASIS AND AXOPLASMIC AMINE CONCENTRATION DETERMINE CALCIUM-INDEPENDENT NORADRENALINE RELEASE IN NORMOXIC AND ISCHEMIC RAT-HEART [J].

SCHOMIG, A ;

KURZ, T ;

RICHARDT, G ;

SCHOMIG, E .

CIRCULATION RESEARCH, 1988, 63 (01) :214-226

[8] Regional myocardial interstitial norepinephrine kinetics during coronary occlusion and reperfusion [J].

Shindo, T ;

Akiyama, T ;

Yamazaki, T ;

Ninomiya, I .

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 1996, 270 (01) :H245-H251

[9] ROUTINE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF MYOCARDIAL INTERSTITIAL NOREPINEPHRINE [J].

YAMAZAKI, T ;

AKIYAMA, T ;

SHINDO, T .

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1995, 670 (02) :328-331

[10] INVIVO MEASUREMENT OF DOPAMINE AND ITS METABOLITES BY INTRACEREBRAL DIALYSIS - CHANGES AFTER D-AMPHETAMINE [J].

ZETTERSTROM, T ;

SHARP, T ;

MARSDEN, CA ;

UNGERSTEDT, U .

JOURNAL OF NEUROCHEMISTRY, 1983, 41 (06) :1769-1773