Comparative Examination of Escherichia coli O157:H7 Survival on Romaine Lettuce and in Soil at Two Independent Experimental Sites

被引:52
作者
Bezanson, Greg [1 ]
Delaquis, Pascal [2 ]
Bach, Susan [2 ]
McKellar, Robin [2 ]
Topp, Ed [3 ]
Gill, Alex [4 ]
Blais, Burton [5 ]
Gilmour, Matthew [6 ]
机构
[1] Agr & Agri Food Canada, Atlantic Food & Hort Res Ctr, Kentville, NS B4N 1J5, Canada
[2] Agr & Agri Food Canada, Pacific Agri Food Res Ctr, Summerland, BC V0H 1Z0, Canada
[3] Agr & Agri Food Canada, So Crop Protect & Food Res Ctr, London, ON N5V 4T3, Canada
[4] Hlth Canada, Bur Microbial Hazards, Sir Frederick Banting Res Ctr, Ottawa, ON K1A 0L2, Canada
[5] Canadian Food Inspect Agcy, Ottawa, ON K2E 8A5, Canada
[6] Publ Hlth Agcy Canada, Natl Microbiol Lab, Winnipeg, MB R3E 3R2, Canada
关键词
SALMONELLA-ENTERICA; CONTAMINATED MANURE; IRRIGATION WATER; FRESH PRODUCE; LEAF LETTUCE; O157-H7; O157H7; PERSISTENCE; COLONIZATION; FRUIT;
D O I
10.4315/0362-028X.JFP-11-306
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Little is known about the influence of abiotic factors such as climate and soil chemistry on the survival of Escherichia coli O157:H7 in field lettuce. We applied a nalidixic acid resistant derivative of strain ATCC 700728 to field-grown romaine lettuce in two regions in Canada characterized by large variances in soil type and climate. Surviving populations in soil and on lettuce leaves were estimated on sorbitol MacConkey agar supplemented with nalidixic acid. Data were fitted with the Weibull decline function to permit comparison of decay rates in the two experimental sites. E. coli O157:H7 populations fell from 10(5) to <10(2) CFU/g on leaves, and <10(3) CFU/g in soil within 7 days after inoculation. Analysis revealed there was no significant difference between decay rates at the two experimental sites in either environment. The results of this study suggest that the inherent ecological fitness of E. coli O157:H7 ATCC 700728 determines the extent of survival in the production environment.
引用
收藏
页码:480 / 487
页数:8
相关论文
共 36 条
[1]  
[Anonymous], [No title captured]
[2]   STARVATION-INDUCED AND STATIONARY-PHASE-INDUCED ACID TOLERANCE IN ESCHERICHIA-COLI O157/H7 [J].
ARNOLD, KW ;
KASPAR, CW .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (05) :2037-2039
[3]   Persistence of Escherichia coli on Injured iceberg Lettuce in the Field, Overhead Irrigated with Contaminated Water [J].
Barker-Reid, Fiona ;
Harapas, Dean ;
Engleitner, Siegfried ;
Kreidl, Simone ;
Holmes, Robert ;
Faggian, Robert .
JOURNAL OF FOOD PROTECTION, 2009, 72 (03) :458-464
[4]   Fresh fruit and vegetables as vehicles for the transmission of human pathogens [J].
Berger, Cedric N. ;
Sodha, Samir V. ;
Shaw, Robert K. ;
Griffin, Patricia M. ;
Pink, David ;
Hand, Paul ;
Frankel, Gad .
ENVIRONMENTAL MICROBIOLOGY, 2010, 12 (09) :2385-2397
[5]   Variation in acid resistance among enterohaemorrhagic Escherichia coli in a simulated gastric environment [J].
Bergholz, T. M. ;
Whittam, T. S. .
JOURNAL OF APPLIED MICROBIOLOGY, 2007, 102 (02) :352-362
[6]  
Brandi M., 2006, ANNU REV PHYTOPATHOL, V44, P17
[7]   Escherichia coli O157:H7 stress and virulence gene expression on Romaine lettuce using comparative real-time PCR [J].
Carey, Christine M. ;
Kostrzynska, Magdalena ;
Thompson, Stacey .
JOURNAL OF MICROBIOLOGICAL METHODS, 2009, 77 (02) :235-242
[8]   Escherichia coli O157:H7 survival and growth on lettuce is altered by the presence of epiphytic bacteria [J].
Cooley, Michael B. ;
Chao, Diana ;
Mandrell, Robert E. .
JOURNAL OF FOOD PROTECTION, 2006, 69 (10) :2329-2335
[9]   Behavior of Escherichia coli O157:H7 in leafy vegetables [J].
Delaquis, Pascal ;
Bach, Susan ;
Dinu, Laura-Dorina .
JOURNAL OF FOOD PROTECTION, 2007, 70 (08) :1966-1974
[10]   Summer meeting 2007 - the problems with fresh produce: an overview [J].
Doyle, M. P. ;
Erickson, M. C. .
JOURNAL OF APPLIED MICROBIOLOGY, 2008, 105 (02) :317-330