Label-free detection of aflatoxin M1 with electrochemical Fe3O4/polyaniline-based aptasensor

被引:128
作者
Binh Hai Nguyen [1 ]
Lam Dai Tran [1 ]
Quan Phuc Do [2 ]
Huy Le Nguyen [3 ]
Ngoc Huan Tran [4 ]
Phuc Xuan Nguyen [1 ]
机构
[1] Vietnam Acad Sci & Technol, Inst Mat Sci, Hanoi, Vietnam
[2] Hanoi Univ Sci, Res Ctr Environm Technol & Sustainable Dev, Hanoi, Vietnam
[3] Hanoi Univ Sci & Technol, Sch Chem Engn, Hanoi, Vietnam
[4] Hanyang Univ, Dept Chem, Seoul 133791, South Korea
来源
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2013年 / 33卷 / 04期
关键词
Fe3O4-doped polyaniline; Aptasensor; Aflatoxin M1 (AFM1); Electrochemical detection; LIQUID-CHROMATOGRAPHY; RAPID DETECTION; M-1; DETECTION; MILK; IMMUNOASSAY; MYCOTOXINS; IMMUNOSENSOR; LIGANDS; ELISA; CONTAMINATION;
D O I
10.1016/j.msec.2013.01.044
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
The selective detection of ultratrace amounts of aflatoxin M1 (AFM1) is extremely important for food safety since it is the most toxic mycotoxin class that is allowed to be present on cow milk with strictly low regulatory levels. In this work, Fe3O4 incorporated polyaniline (Fe3O4/PANi) film has been polymerized on interdigitated electrode (IDE) as sensitive film for AFM1 electrochemical biosensor. The immobilized aptamers as an affinity capture reagent and magnetic nanoparticles for signal amplification element have been employed in the sensing platform. Label-free and direct detection of the aptamer-AFM1 on Fe3O4/PANi interface were performed via electrochemical signal change, acquired by cyclic and square wave voltammetries. With a simplified strategy, this electrochemical aptasensor shows a good sensitivity to AFM1 in the range of 6-60 ng.L-1, with the detection limit of 1.98 ng.L-1. The results open up the path for designing cost effective aptasensors for other biomedical applications. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:2229 / 2234
页数:6
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