Dynamic regulation of uncoupling protein 2 content in INS-1E insulinoma cells

被引：38

作者：

Azzu, Vian ^{[1
]}

Affourtit, Charles ^{[1
]}

Breen, Eamon P. ^{[1
]}

Parker, Nadeene ^{[1
]}

Brand, Martin D. ^{[1
]}

机构：

[1] MRC, Dunn Human Nutr Unit, Cambridge CB2 0XY, England

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 2008年 / 1777卷 / 10期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

Glutamine; Half-life; Mitochondria; Pancreatic beta-cell; Serum starvation; UCP2;

D O I：

10.1016/j.bbabio.2008.07.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Uncoupling protein 2 (UCP2) regulates glucose-stimulated insulin secretion in pancreatic beta-cells. UCP2 content, measured by calibrated immunoblot in INS-1E insulinoma cells (a pancreatic beta-cell model) grown in RPMI medium, and INS-1E mitochondria, was 2.0 ng/million cells (7.9 ng/mg mitochondrial protein). UCP2 content was lower in cells incubated without glutamine and higher in cells incubated with 20 mM glucose, and varied from 1.0-4.4 ng/million cells (2.7-14.5 ng/mg mitochondrial protein). This dynamic response to nutrients was achieved by varied expression rates against a background of a very short UCP2 protein half-life of about 1 h. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1378 / 1383

页数：6

共 31 条

[1] Stronger control of ATP/ADP by proton leak in pancreatic β-cells than skeletal muscle mitochondria
Affourtit, C
Brand, MD
[J]. BIOCHEMICAL JOURNAL, 2006, 393 : 151 - 159
[2] AFFOURTIT C, 2008, BIOCHIM BIOPHYS ACTA, DOI DOI 10.1016/J.BBABIO.2008.1003.1022
[3] Uncoupling protein-2 contributes significantly to high mitochondrial proton leak in INS-1E insulinoma cells and attenuates glucose-stimulated insulin secretion
Affourtit, Charles
Brand, Martin D.
[J]. BIOCHEMICAL JOURNAL, 2008, 409 (199-204) : 199 - 204
[4] ESTABLISHMENT OF 2-MERCAPTOETHANOL-DEPENDENT DIFFERENTIATED INSULIN-SECRETING CELL-LINES
ASFARI, M
JANJIC, D
MEDA, P
LI, GD
HALBAN, PA
WOLLHEIM, CB
[J]. ENDOCRINOLOGY, 1992, 130 (01) : 167 - 178
[5] Sirt1 regulates insulin secretion by repressing UCP2 in pancreatic β cells
Bordone, L
Motta, MC
Picard, F
Robinson, A
Jhala, US
Apfeld, J
McDonagh, T
Lemieux, M
McBurney, M
Szilvasi, A
Easlon, EJ
Lin, SJ
Guarente, L
[J]. PLOS BIOLOGY, 2006, 4 (02): : 210 - 220
[6] Glucose induces and leptin decreases expression of uncoupling protein-2 mRNA in human islets
Brown, JEP
Thomas, S
Digby, JE
Dunmore, SJ
[J]. FEBS LETTERS, 2002, 513 (2-3) : 189 - 192
[7] Increased uncoupling protein-2 levels in β-cells are associated with impaired glucose-stimulated insulin secretion -: Mechanism of action
Chan, CB
De Leo, D
Joseph, JW
McQuaid, TS
Ha, XF
Xu, F
Tsushima, RG
Pennefathner, PS
Salapatek, AMF
Wheeler, MB
[J]. DIABETES, 2001, 50 (06) : 1302 - 1310
[8] Overexpression of uncoupling protein 2 inhibits glucose-stimulated insulin secretion from rat islets
Chan, CB
MacDonald, PE
Saleh, MC
Johns, DC
Marbàn, E
Wheeler, MB
[J]. DIABETES, 1999, 48 (07) : 1482 - 1486
[9] Inhibition of UCP2 expression reverses diet-induced diabetes mellitus by effects on both insulin secretion and action
De Souza, Claudio T.
Araujo, Eliana P.
Stoppiglia, Luiz F.
Pauli, Jose R.
Ropelle, Eduardo
Rocco, Silvana A.
Marin, Rodrigo M.
Franchini, Kleber G.
Carvalheira, Jose B.
Saad, Mario J.
Boschero, Antonio C.
Carneiro, Everardo M.
Velloso, Licio A.
[J]. FASEB JOURNAL, 2007, 21 (04) : 1153 - 1163
[10] Peroxisome proliferator-activated receptor γ coactivator-1-dependent uncoupling protein-2 expression in pancreatic islets of rats:: a novel pathway for neural control of insulin secretion
De Souza, CT
Gasparetti, AL
Pereira-da-Silva, M
Araújo, EP
Carvalheira, JB
Saad, MJA
Boschero, AC
Carneiro, EM
Velloso, LA
[J]. DIABETOLOGIA, 2003, 46 (11) : 1522 - 1531

← 1 2 3 4 →