Three-dimensional spheroid model in tumor biology

被引:228
作者
Santini, MT
Rainaldi, G
机构
[1] Ist Super Sanita, Ultrastrutture Lab, I-00161 Rome, Italy
[2] Univ Naples Federico II, INFM, Unita Napoli, Naples, Italy
关键词
multicellular spheroids; tumors; cell death;
D O I
10.1159/000028065
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
It is becoming more and more apparent that monolayer cultures of tumor cells cannot completely represent the characteristics of three-dimensional solid tumors. Consequently, the multicellular tumor spheroid model, which is of intermediate complexity between in vivo tumors and monolayer cultures, was developed. In this review,the major similarities between spheroids and solid tumors are discussed. After a brief survey of the different spheroid culturing techniques, the general morphological and growth characteristics of these systems are examined and compared to solid tumors. Finally, selected studies regarding the use of tumor spheroids to examine cell response to antineoplastic agents and radiation, cell death including both necrosis and apoptosis and cell adhesion in spheroids are reviewed.
引用
收藏
页码:148 / 157
页数:10
相关论文
共 65 条
[1]  
[Anonymous], 1996, Ackerman's surgical pathology
[2]   Clinical relevance of transmembrane drug efflux as a mechanism of multidrug resistance [J].
Bradshaw, DM ;
Arceci, RJ .
JOURNAL OF CLINICAL ONCOLOGY, 1998, 16 (11) :3674-3690
[3]  
CARLSSON J, 1984, RECENT RESULTS CANC, V95, P1
[4]  
CONGER AD, 1983, CANCER RES, V43, P556
[5]  
Dangles V, 1997, CANCER RES, V57, P3360
[6]  
DEMICHELI R, 1989, CANCER RES, V49, P6543
[7]   THE EXPONENTIAL-GOMPERTZIAN TUMOR-GROWTH MODEL - DATA FROM 6 TUMOR-CELL LINES INVITRO AND INVIVO - ESTIMATE OF THE TRANSITION POINT FROM EXPONENTIAL TO GOMPERTZIAN GROWTH AND POTENTIAL CLINICAL IMPLICATIONS [J].
DEMICHELI, R ;
PRATESI, G ;
FORONI, R .
TUMORI JOURNAL, 1991, 77 (03) :189-195
[8]   INCREASED RADIORESISTANCE OF CELLS IN CULTURED MULTICELL SPHEROIDS .1. DEPENDENCE ON CELLULAR INTERACTION [J].
DERTINGER, H ;
HULSER, D .
RADIATION AND ENVIRONMENTAL BIOPHYSICS, 1981, 19 (02) :101-107
[9]   Upregulation of interleukin 8 by oxygen-deprived cells in glioblastoma suggests a role in leukocyte activation, chemotaxis, and angiogenesis [J].
Desbaillets, I ;
Diserens, AC ;
deTribolet, N ;
Hamou, MF ;
Van Meir, EG .
JOURNAL OF EXPERIMENTAL MEDICINE, 1997, 186 (08) :1201-1212
[10]   Cancer: A challenge for control theory and computer modelling [J].
Duchting, W ;
Ulmer, W ;
Ginsberg, T .
EUROPEAN JOURNAL OF CANCER, 1996, 32A (08) :1283-1292