Nucleic acid-functionalized Pt nanoparticles: Catalytic labels for the amplified electrochemical detection of biomolecules

被引：408

作者：

Polsky, R ^{[1
]}

Gill, R ^{[1
]}

Kaganovsky, L ^{[1
]}

Willner, I ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel

来源：

ANALYTICAL CHEMISTRY | 2006年 / 78卷 / 07期

关键词：

D O I：

10.1021/ac0519864

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Nucleic acid-functionalized Pt nanoparticles (Pt-NPs) act as catalytic labels for the amplified electrochemical detection of DNA hybridization and aptamer/protein recognition. Hybridization of the nucleic acid-modified Pt-NPs with a sensing nucleic acid/analyte DNA complex associated with an electrode enables the amperometric, amplified, detection of the DNA by the Pt NP electrocatalyzed reduction of H2O2 (sensitivity limit, 1 x 10(-11) M). Similarly, the association of aptamer-functionalized Pt-NPs to a thrombin aptamer/thrombin complex associated with an electrode allowed the amplified, electrocatalytic detection of thrombin with a sensitivity limit corresponding to 1 x 10(-9) M.

引用

页码：2268 / 2271

页数：4

共 21 条

[1] SELECTION OF SINGLE-STRANDED-DNA MOLECULES THAT BIND AND INHIBIT HUMAN THROMBIN [J].

BOCK, LC ;

GRIFFIN, LC ;

LATHAM, JA ;

VERMAAS, EH ;

TOOLE, JJ .

NATURE, 1992, 355 (6360) :564-566

[2] Electrochemical DNA sensors [J].

Drummond, TG ;

Hill, MG ;

Barton, JK .

NATURE BIOTECHNOLOGY, 2003, 21 (10) :1192-1199

[3] INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS [J].

ELLINGTON, AD ;

SZOSTAK, JW .

NATURE, 1990, 346 (6287) :818-822

[4]

Gooding JJ, 2002, ELECTROANAL, V14, P1149, DOI 10.1002/1521-4109(200209)14:17<1149::AID-ELAN1149>3.0.CO

[5]

2-8

[6] Integrated nanoparticle-biomolecule hybrid systems: Synthesis, properties, and applications [J].

Katz, E ;

Willner, I .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (45) :6042-6108

[7]

Park SJ, 2002, SCIENCE, V295, P1503, DOI 10.1126/science.1067003

[8]

Patolsky F, 2000, ANGEW CHEM INT EDIT, V39, P940, DOI 10.1002/(SICI)1521-3773(20000303)39:5<940::AID-ANIE940>3.3.CO

[9]

2-P

[10] Enzyme-linked amplified electrochemical sensing of oligonucleotide-DNA interactions by means of the precipitation of an insoluble product and using impedance spectroscopy [J].

Patolsky, F ;

Katz, E ;

Bardea, A ;

Willner, I .

LANGMUIR, 1999, 15 (11) :3703-3706

← 1 2 3 →