Mild Cerebellar Neurodegeneration of Aged Heterozygous PCD Mice Increases Cell Fusion of Purkinje and Bone Marrow-Derived Cells

被引:16
作者
Diaz, David [1 ,2 ]
Recio, Javier S. [1 ,2 ]
Weruaga, Eduardo [1 ,2 ]
Alonso, Jose R. [1 ,2 ,3 ]
机构
[1] Univ Salamanca, Inst Neurosci Castile & Leon, Lab Neuronal Plast & Neurorepair, E-37008 Salamanca, Spain
[2] Univ Salamanca, Inst Biomed Res Salamanca, E-37008 Salamanca, Spain
[3] Univ Tarapaca, Inst Alta Invest, Arica, Chile
关键词
Cell fusion; Cerebellum; Neurorepair; Purkinje cell degeneration; Purkinje cells; Transplant; MESENCHYMAL STEM-CELLS; SPINAL-CORD-INJURY; DEGENERATION PCD; HEMATOPOIETIC-CELLS; OLFACTORY-BULB; STROMAL CELLS; MUTANT MOUSE; NEURONS; TRANSPLANTATION; SURVIVAL;
D O I
10.3727/096368912X638900
中图分类号
Q813 [细胞工程];
学科分类号
100113 [医学细胞生物学];
摘要
Bone marrow-derived cells have different plastic properties, especially regarding cell fusion, which increases with time and is prompted by tissue injury. Several recessive mutations, including Purkinje Cell Degeneration, affect the number of Purkinje cells in homozygosis; heterozygous young animals have an apparently normal phenotype but they undergo Purkinje cell loss as they age. Our findings demonstrate that heterozygous pcd mice undergo Purkinje cell loss at postnatal day 300, this slow but steadily progressing cell death starting sooner than has been reported previously and without massive reactive gliosis or inflammation. Here, transplantation of bone marrow stem cells was performed to assess the arrival of bone marrow-derived cells in the cerebellum in these heterozygous mice. Our results reveal that a higher number of cell fusion events occurs in heterozygous animals than in the controls, on days 150 and 300 postnatally. In sum, this study indicates that mild cell death promotes the fusion of bone marrow-derived cells with surviving Purkinje neurons. This phenomenon suggests new therapies for long-lasting neurodegenerative disorders.
引用
收藏
页码:1595 / 1602
页数:8
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