Effects of cadmium on antioxidant enzyme activities in sugar cane

被引：128

作者：

Fornazier, RF

Ferreira, RR

Vitória, AP

Molina, SMG

Lea, PJ

Azevedo, RA ^{[1
]}

机构：

[1] Univ Sao Paulo, Escola Superior Agr Luiz Queiroz, Dept Genet, BR-13400970 Piracicaba, SP, Brazil

[2] Univ Lancaster, Dept Sci Biol, Lancaster LA1 4YQ, England

来源：

BIOLOGIA PLANTARUM | 2002年 / 45卷 / 01期

关键词：

catalase; glutathione reductase; heavy metals; phytochelatins; reactive oxygen species; Saccharum officinarum; superoxide dismutase;

D O I：

10.1023/A:1015100624229

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Sugar cane (Saccharum officinarum L, cv. Copersucar SP80-3280) seedlings were grown in nutrient solution with varying concentrations (0, 2 and 5 mM) of cadmium chloride for 96 h. Leaves were analysed for catalase (CAT), glutathione reductase (GR) and superoxide dismutase (SOD) activities. Although a clear effect of CdCl2 on plant growth was observed, the activity of SOD was riot altered significantly. However, the CAT activity decreased as the concentration of CdCl2 increased. GR exhibits a significant increase in activity at 2 and 5 mM CdCl2. CAT and SOD isoenzymes were further characterised by analysis in non-denaturing PAGE. Activity staining for SOD revealed up to seven isoenzymes in untreated control and 2 mM CdCl2 treated plants, corresponding to Cu/Zn-SOD isoenzymes. At 5 mM CdCl2, only six Cu/Zn-SOD isoenzymes were observed. No Fe-SOD and Mn-SOD isoenzymes were detected. For CAT, one band of activity was observed.

引用

页码：91 / 97

页数：7

共 48 条

[1] Responses to cadmium in leaves of transformed poplars overexpressing γ-glutamylcysteine synthetase [J].