Co-culture of hepatocytes and fibroblasts by micropatterned immobilization of β-galactose derivatives

被引：49

作者：

Kang, IK ^{[1
]}

Kim, GJ

Kwon, OH

Ito, Y

机构：

[1] Kyungpook Natl Univ, Dept Polymer Sci, Taegu 702701, South Korea

[2] Kumoh Natl Univ, Dept Polymer Sci & Engn, Kumi 730701, South Korea

[3] Kanagawa Acad Sci & Technol, Kawasaki, Kanagawa 2130012, Japan

来源：

BIOMATERIALS | 2004年 / 25卷 / 18期

基金：

新加坡国家研究基金会;

关键词：

poly(allylamine); micropatterned surface; hepatocyte; co-culture;

D O I：

10.1016/j.biomaterials.2003.11.004

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Photosensitive poly(allylamine) containing beta-galactose moieties in the side chain (LPAN(3)) was prepared by the reaction of poly (allyl amine) with lactobionic acid and azidobenzoic acid. To create micropatterned surfaces, a LPAN(3)-coated poly(methyl methacrylate) (PMMA) substrate was irradiated with an ultraviolet lamp under a photomask. The presence of a LPAN(3) layer on the substrate was confirmed using an electron spectroscopy for chemical analysis. Micropatterned cell culture was carried out by seeding hepatocytes and/or fibroblasts on the substrate. As a result, hepatocytes and fibroblasts adhered only to the LPAN(3) and PMMA lane, respectively. Co-culture on the stripe patterned substrate was carried out by the first cell seeding of hepatocytes and subsequently by the second cell seeding of fibroblasts. The co-cultured cells produced extracellular matrix such as fibronectin, suggesting a biological function. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：4225 / 4232

页数：8

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