Human monocyte response to cementum extracts from periodontally diseased teeth: Effect of conditioning with tetracycline

被引:9
作者
Shapira, L
Houri, Y
Barak, V
Halabi, A
Soskolne, WA
Stabholz, A
机构
[1] HADASSAH MED CTR,DEPT ONCOL,IL-91120 JERUSALEM,ISRAEL
[2] HEBREW UNIV JERUSALEM,HADASSAH FAC DENT MED,DEPT COMMUNITY DENT,JERUSALEM,ISRAEL
关键词
periodontal diseases/pathogenesis; cementum; cytokines; monocytes; tetracycline therapeutic use; endotoxins;
D O I
10.1902/jop.1996.67.7.682
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
MONOCYTE INFLAMMATORY CYTOKINES, such as TNF alpha and IL-1 beta, have been implicated in the pathogenesis of periodontal destruction. The present study was designed to test the ability of extracts of cementum from periodontally diseased teeth to induce the secretion of these mediators by monocytes, to evaluate the role of adsorbed endotoxin in this process, and to test the effect of cementum conditioning with tetracycline on the monocyte response. Human monocytes were incubated with varying concentrations of cementum extracts, and TNF alpha and IL-1 beta levels in the media were measured. The results showed that while extracts of healthy cementum had no effect on monocyte secretion, concentration as low as 0.5 mg/ml of cementum from diseased sites raised the levels of TNF alpha and IL-1 beta secretion 10-fold. This response was dose-dependent. Diseased cementum were found to contain 1.5 ng/mg endotoxin, while endotoxin was not detectable in the extracts of the healthy cementum. However, neutralization of the endotoxin by polymyxin B only partially reduced the monocyte secretory response by 50 to 70%, suggesting that other factors in the extracts are also involved in monocyte stimulation. To simulate the effect of root conditioning, cementum was first agitated in a tetracycline or control solution prior to its extraction in media. Pretreatment of diseased cementum with tetracycline (50 mg/ml) was found to block the secretion of TNF alpha from cementum-stimulated monocytes. Pretreatment of the diseased cementum with 10 mg/ml tetracycline was not more effective than saline and HCl controls, with all treatments reducing cytokine secretion by approximate to 80%. The direct addition of tetracycline to cementum-stimulated monocyte culture was found to block TNF alpha secretion in a dose dependent manner. The results suggest that extracts from diseased cementum are potent stimulators of monocyte secretion, and that endotoxin as well as other factor(s) appear to be involved. These factors are partially extracted by washing and a 10 mg/ml tetracycline solution is not more effective than saline in achieving this goal. In addition, tetracycline was found to be a potent inhibitor of TNF alpha secretion by cementum-stimulated monocytes, suggesting a novel mechanism for this drug in periodontal therapy.
引用
收藏
页码:682 / 687
页数:6
相关论文
共 38 条
  • [1] PRESENCE AND BIOLOGIC ACTIVITY OF CEMENTUM-BOUND ENDOTOXIN
    ALEO, JJ
    DERENZIS, FA
    FARBER, PA
    VARBONCOEUR, AP
    [J]. JOURNAL OF PERIODONTOLOGY, 1974, 45 (09) : 672 - 675
  • [2] BJORVATN K, 1985, SCAND J DENT RES, V93, P192
  • [3] BJORVATN K, 1983, SCAND J DENT RES, V91, P417
  • [4] THE PATHOGENESIS OF SEPSIS
    BONE, RC
    [J]. ANNALS OF INTERNAL MEDICINE, 1991, 115 (06) : 457 - 469
  • [5] TETRACYCLINES INHIBIT PORPHYROMONAS GINGIVALIS-INDUCED ALVEOLAR BONE LOSS IN RATS BY A NONANTIMICROBIAL MECHANISM
    CHANG, KM
    RAMAMURTHY, NS
    MCNAMARA, TF
    EVANS, RT
    KLAUSEN, B
    MURRAY, PA
    GOLUB, LM
    [J]. JOURNAL OF PERIODONTAL RESEARCH, 1994, 29 (04) : 242 - 249
  • [6] ASSESSMENT OF ULTRASONIC DEBRIDEMENT OF CALCULUS-ASSOCIATED PERIODONTALLY-INVOLVED ROOT SURFACES BY THE LIMULUS AMEBOCYTE LYSATE ASSAY - AN INVITRO STUDY
    CHIEW, SYT
    WILSON, M
    DAVIES, EH
    KIESER, JB
    [J]. JOURNAL OF CLINICAL PERIODONTOLOGY, 1991, 18 (04) : 240 - 244
  • [7] ENDOTOXIN LEVELS IN PERIODONTALLY HEALTHY AND DISEASED SITES - CORRELATION WITH LEVELS OF GRAM-NEGATIVE BACTERIA
    FINE, DH
    MENDIETA, C
    BARNETT, ML
    FURGANG, D
    NAINI, A
    VINCENT, JW
    [J]. JOURNAL OF PERIODONTOLOGY, 1992, 63 (11) : 897 - 901
  • [8] PRELIMINARY CHARACTERIZATION OF MATERIAL ELUTED FROM THE ROOTS OF PERIODONTALLY DISEASED TEETH
    FINE, DH
    MORRIS, ML
    TABAK, L
    COLE, JD
    [J]. JOURNAL OF PERIODONTAL RESEARCH, 1980, 15 (01) : 10 - 19
  • [9] TETRACYCLINES INHIBIT CONNECTIVE-TISSUE BREAKDOWN - NEW THERAPEUTIC IMPLICATIONS FOR AN OLD FAMILY OF DRUGS
    GOLUB, LM
    RAMAMURTHY, NS
    MCNAMARA, TF
    GREENWALD, RA
    RIFKIN, BR
    [J]. CRITICAL REVIEWS IN ORAL BIOLOGY AND MEDICINE, 1991, 2 (03) : 297 - 322
  • [10] GRAM-POSITIVE CELL-WALLS STIMULATE SYNTHESIS OF TUMOR-NECROSIS-FACTOR-ALPHA AND INTERLEUKIN-6 BY HUMAN MONOCYTES
    HEUMANN, D
    BARRAS, C
    SEVERIN, A
    GLAUSER, MP
    TOMASZ, A
    [J]. INFECTION AND IMMUNITY, 1994, 62 (07) : 2715 - 2721