Identification of AFLP markers closely linked to the powdery mildew resistance genes Pm1c and Pm4a in common wheat (Triticum aestivum L.)

A total of 7654 DNA fragments were screened for linkage to wheat powdery mildew resistance gene Pmlc employing fluorescently based AFLP analysis and phenotypic pools from F-3 families. F-3 and derived F-4 families were used for segregation analysis. Pool screening revealed several cosegregating and tightly linked (0.9 cM) AFLP markers for the Pmlc resistance gene. The previously reported RFLP locus Xwhs178 was integrated into the AFLP map in the vicinity of Pmlc. One AFLP marker, 18M2, was determined to be highly specific for the Pmlc gene in diverse genetic backgrounds. As Pmlc allele confers an effective resistance to powdery mildew, the marker 18M2 provides a valuable tool for enhancing marker assisted selection and pyramiding of powdery mildew resistance genes in wheat.