Neospora caninum and Waddlia chondrophila strain 2032/99 in a septic stillborn calf

被引：80

作者：

Henning, K

Schares, G

Granzow, H

Polster, U

Hartmann, M

Hotzel, H

Sachse, K

Peters, M

Rauser, M

机构：

[1] Fed Res Ctr Virus Dis Anim, D-16868 Wusterhausen, Germany

[2] Fed Res Ctr Virus Dis Anim, D-17598 Insel Riems, Germany

[3] Klinikum Friedrich Schiller Univ, Inst Med Mikrobiol, D-07743 Jena, Germany

[4] Fed Inst Hlth Protect Consumers & Vet Med, D-07743 Jena, Germany

[5] Tierarztlichen Hsch Hannover, Inst Pathol, D-30559 Hannover, Germany

[6] Tierarztpraxis M Rauser, D-56283 Halsenbach, Germany

来源：

VETERINARY MICROBIOLOGY | 2002年 / 85卷 / 03期

关键词：

cattle bacterial; bacterial abortion; intracellular micro-organism; Chlamydia; Waddlia chondrophila; antibiotic sensitivity; electron microscopy; DNA sequencing;

D O I：

10.1016/S0378-1135(01)00510-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Characteristics of an intracellularly growing micro-organism isolated from an aborted bovine foetus are described. The organism replicated within cytoplasmic vacuoles, was resistant to penicillin and exhibited structural characteristics compatible with Waddlia chondrophila. An ELISA specific for Chlamydia spp., immunofluorescence tests using antibodies directed against Chlamydia spp. or Simkania negevensis, and PCR using Chlamydia-specific primers showed that the agent was distinct from Chlamydiae or S. negevensis. Determination of 16S and partial 23S ribosomal RNA gene sequences in combination with the PCR results and the morphological, antigenic and developmental characteristics provided evidence that the isolate 2032/99 can be classified as W chondrophila or a closely related organism. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：285 / 292

页数：8

共 17 条

[1]

Ahlers D, 1997, PRAKT TIERARZT, V78, P674

[2] ISOLATION OF A PREVIOUSLY UNDESCRIBED RICKETTSIA FROM AN ABORTED BOVINE FETUS [J].