Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine

被引：76

作者：

Araya, Roberto ^{[2
]}

Andino-Pavlovsky, Victoria ^{[1
]}

Yuste, Rafael ^{[2
]}

Etchenique, Roberto ^{[1
]}

机构：

[1] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Inorgan Analit & Quim Fis, INQUIMAE, Buenos Aires, DF, Argentina

[2] Columbia Univ, Howard Hughes Med Inst, Dept Biol Sci, New York, NY 10027 USA

来源：

ACS CHEMICAL NEUROSCIENCE | 2013年 / 4卷 / 08期

关键词：

Dopamine; ruthenium; two-photon; caged compound; PREFRONTAL CORTEX; RECEPTORS; D-1; LOCALIZATION; LIGHT; POTENTIALS; MODULATION; ACTIVATION; MICROSCOPY; COMPLEXES;

D O I：

10.1021/cn4000692

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We introduce a novel caged dopamine compound (RuBi-Dopa) based on ruthenium photochemistry. RuBi-Dopa has a high uncaging efficiency and can be released with visible (blue-green) and IR light in a two-photon regime. We combine two-photon photorelease of RuBi-Dopa with two-photon calcium imaging for an optical imaging and manipulation of dendritic spines in living brain slices, demonstrating that spines can express functional dopamine receptors. This novel compound allows mapping of functional dopamine receptors in living brain tissue with exquisite spatial resolution.

引用

页码：1163 / 1167

页数：5

共 31 条

[1] Neuroleptic malignant syndrome [J].