Functional characterization of DNAM-1 (CD226) interaction with its ligands PVR (CD155) and nectin-2 (PRR-2/CD112)

被引:223
作者
Tahara-Hanaoka, S
Shibuya, K
Onoda, Y
Zhang, H
Yamazaki, S
Miyamoto, A
Honda, S
Lanier, LL
Shibuya, A
机构
[1] RIKEN, Res Ctr Allergy & Immunol, Lab Immune Receptor, Tsukuba, Ibaraki 3050074, Japan
[2] Univ Tsukuba, Inst Basic Med Sci, Dept Immunol, Tsukuba, Ibaraki 3058575, Japan
[3] Japan Sci & Technol Agcy, PRESTO, Saitama 3320012, Japan
[4] Univ Calif San Francisco, Dept Microbiol & Immunol, San Francisco, CA 94143 USA
[5] Univ Calif San Francisco, Canc Res Inst, San Francisco, CA 94143 USA
关键词
adhesion molecule; cytotoxic T lymphocyte; cytotoxicity; NK cell; tumor immunity;
D O I
10.1093/intimm/dxh059
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
CD226 (DNAM-1) is an adhesion molecule involved in NK and T cell-mediated cytotoxicity against certain tumors. Here, we have identified the human poliovirus receptor-related (PRR) family members CD155 [poliovirus receptor (PVR)] and CD112 (nectin-2/PRR-2) as the ligands for human CD226. Ectopic expression of human CD155 and/or CD112 rendered mouse BW5147 T cells more susceptible to IL-2-activated T and NK cell-mediated cytotoxicity, and killing was specifically inhibited by anti-CD226 mAb, demonstrating functional interactions of CD226 with CD155 and CD112. Although the binding affinities between soluble CD226 and CD155 or CD112 were comparable, the homophilic interaction of cell-surface CD112 may adversely affect CD226 binding to CD112. We also demonstrate that ligation of CD226 and LFA-1 with their respective ligands cooperates in triggering cytotoxicity and cytokine secretion by T and NK cells.
引用
收藏
页码:533 / 538
页数:6
相关论文
共 16 条
[1]   Mouse homolog of poliovirus receptor-related gene 2 product, mPRR2, mediates homophilic cell aggregation [J].
Aoki, J ;
Koike, S ;
Asou, H ;
Ise, I ;
Suwa, H ;
Tanaka, T ;
Miyasaka, M ;
Nomoto, A .
EXPERIMENTAL CELL RESEARCH, 1997, 235 (02) :374-384
[2]   Identification of PVR (CD155) and nectin-2 (CD112) as cell surface ligands for the human DNAM-1 (CD226) activating molecule [J].
Bottino, C ;
Castriconi, R ;
Pende, D ;
Rivera, P ;
Nanni, M ;
Carnemolla, B ;
Cantoni, C ;
Grassi, J ;
Marcenaro, S ;
Reymond, N ;
Vitale, M ;
Moretta, L ;
Lopez, M ;
Moretta, A .
JOURNAL OF EXPERIMENTAL MEDICINE, 2003, 198 (04) :557-567
[3]  
CAYABYAB M, 1994, J IMMUNOL, V152, P1523
[4]   ROLE OF LYMPHOCYTE ADHESION RECEPTORS IN TRANSIENT INTERACTIONS AND CELL LOCOMOTION [J].
DUSTIN, ML ;
SPRINGER, TA .
ANNUAL REVIEW OF IMMUNOLOGY, 1991, 9 :27-66
[5]   THE POLIOVIRUS RECEPTOR PROTEIN IS PRODUCED BOTH AS MEMBRANE-BOUND AND SECRETED FORMS [J].
KOIKE, S ;
HORIE, H ;
ISE, I ;
OKITSU, A ;
YOSHIDA, M ;
IIZUKA, N ;
TAKEUCHI, K ;
TAKEGAMI, T ;
NOMOTO, A .
EMBO JOURNAL, 1990, 9 (10) :3217-3224
[6]   CD226 mediates platelet and megakaryocytic cell adhesion to vascular endothelial cells [J].
Kojima, H ;
Kanada, H ;
Shimizu, S ;
Kasama, E ;
Shibuya, K ;
Nakauchi, H ;
Nagasawa, T ;
Shibuya, A .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (38) :36748-36753
[7]   The human poliovirus receptor related 2 protein is a new hematopoietic/endothelial homophilic adhesion molecule [J].
Lopez, M ;
Aoubala, M ;
Jordier, F ;
Isnardon, D ;
Gomez, S ;
Dubreuil, P .
BLOOD, 1998, 92 (12) :4602-4611
[8]   Nectin2α (PRR2α or HveB) and nectin2δ are low-efficiency mediators for entry of herpes simplex virus mutants carrying the Leu25Pro substitution in glycoprotein D [J].
Lopez, M ;
Cocchi, F ;
Menotti, L ;
Avitabile, E ;
Dubreuil, P ;
Campadelli-Fiume, G .
JOURNAL OF VIROLOGY, 2000, 74 (03) :1267-1274
[9]   CELLULAR RECEPTOR FOR POLIOVIRUS - MOLECULAR-CLONING, NUCLEOTIDE-SEQUENCE, AND EXPRESSION OF A NEW MEMBER OF THE IMMUNOGLOBULIN SUPERFAMILY [J].
MENDELSOHN, CL ;
WIMMER, E ;
RACANIELLO, VR .
CELL, 1989, 56 (05) :855-865
[10]   Nectin4/PRR4, a new afadin-associated member of the nectin family that trans-interacts with Nectin1/PRR1 through V domain interaction [J].
Reymond, N ;
Fabre, S ;
Lecocq, E ;
Adelaïde, J ;
Dubreuil, P ;
Lopez, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (46) :43205-43215