Dimethyl sulfoxide as an inducer of differentiation in preosteoblast MC3T3-E1 cells

被引：35

作者：

Cheung, WMW ^{[1
]}

Ng, WW ^{[1
]}

Kung, AWC ^{[1
]}

机构：

[1] Univ Hong Kong, Queen Mary Hosp, Dept Med, Hong Kong, Hong Kong, Peoples R China

来源：

FEBS LETTERS | 2006年 / 580卷 / 01期

关键词：

DMSO; protein kinase C; PKD; erk; proliferation; osteoblasts;

D O I：

10.1016/j.febslet.2005.11.062

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Osteoblastic differentiation is an essential part of bone formation. Dimethyl sulfoxide (DMSO) is a water miscible solvent that is used extensively for receptor ligands in osteoblast studies. However, little is known about its effects on osteoblastogenic precursor cells. In this study, we have used a murine preosteoblast cell line MC3T3-E1 cells to demonstrate that DMSO effectively induces osteoblastic differentiation of MC3T3-E1 cells via the activation of Runx2 and osterix and is dependent upon the protein kinase C (PKC) pathways. We further demonstrated that prolonged activation of PKC pathways is sufficient to induce osteoblastic differentiation, possibly via the activation of PKD/PKCp. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：121 / 126

页数：6

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