Layer-by-layer deposition of avidin and biotin-labeled antibody an a solid surface to prepare a multilayer array of antibody

被引：12

作者：

Hoshi, T ^{[1
]}

Saiki, H ^{[1
]}

Anzai, J ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Pharmaceut Sci, Aoba Ku, Sendai, Miyagi 9808578, Japan

来源：

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2 | 1999年 / 07期

关键词：

D O I：

10.1039/a902951f

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Multilayer thin films containing an antibody have been prepared successfully by depositing avidin and biotinlabeled antibody alternately and repeatedly on the surface of a quartz slide, in which the antibody retains its binding activity.

引用

页码：1293 / 1294

页数：2

共 10 条

[1] Layer-by-layer construction of enzyme multilayers on an electrode for the preparation of glucose and lactate sensors: Elimination of ascorbate interference by means of an ascorbate oxidase multilayer [J].

Anzai, J ;

Takeshita, H ;

Kobayashi, Y ;

Osa, T ;

Hoshi, T .

ANALYTICAL CHEMISTRY, 1998, 70 (04) :811-817

[2] Alternate deposition of concanavalin A and mannose-labelled enzymes on a solid surface to prepare catalytically active enzyme thin films [J].

Anzai, J ;

Kobayashi, Y ;

Nakamura, N .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2, 1998, (03) :461-462

[3] From homogeneous electroenzymatic kinetics to antigen-antibody construction and characterization of spatially ordered catalytic enzyme assemblies on electrodes [J].

Bourdillon, C ;

Demaille, C ;

Moiroux, J ;

Saveant, JM .

ACCOUNTS OF CHEMICAL RESEARCH, 1996, 29 (11) :529-535

[4] Immobilisation of multilayer bioreceptor assemblies on solid substrates [J].

Brynda, E ;

Houska, M ;

Skvor, J ;

Ramsden, JJ .

BIOSENSORS & BIOELECTRONICS, 1998, 13 (02) :165-172

[5]

CARUSO F, 1997, LANGMUIR, V13, P3433

[6] Molecular assembly of multilayer enzyme: Toward the development of a chemiluminescence-based fiber optic biosensor [J].

Chen, ZP ;

Kaplan, DL ;

Gao, H ;

Kumar, J ;

Marx, KA ;

Tripathy, SK .

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC MATERIALS SENSORS AND SYSTEMS, 1996, 4 (03) :155-159

[7] THERMODYNAMICS OF BINDING OF BIOTIN AND SOME ANALOGUES BY AVIDIN [J].

GREEN, NM .

BIOCHEMICAL JOURNAL, 1966, 101 (03) :774-&

[8] ASSEMBLY OF MULTICOMPONENT PROTEIN FILMS BY MEANS OF ELECTROSTATIC LAYER-BY-LAYER ADSORPTION [J].

LVOV, Y ;

ARIGA, K ;

ICHINOSE, I ;

KUNITAKE, T .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (22) :6117-6123

[9] GLUCOSE AND ACETYLCHOLINE SENSING MULTILAYER ENZYME ELECTRODES OF CONTROLLED ENZYME LAYER THICKNESS [J].

RIKLIN, A ;

WILLNER, I .

ANALYTICAL CHEMISTRY, 1995, 67 (22) :4118-4126

[10] THE AVIDIN BIOTIN COMPLEX IN BIOANALYTICAL APPLICATIONS [J].

WILCHEK, M ;

BAYER, EA .

ANALYTICAL BIOCHEMISTRY, 1988, 171 (01) :1-32

← 1 →