High-performance liquid-chromatographic determination of rifampicin in plasma and tissues

被引：51

作者：

Calleja, I ^{[1
]}

Blanco-Príeto, MJ ^{[1
]}

Ruz, N ^{[1
]}

Renedo, MJ ^{[1
]}

Dios-Viéitez, MC ^{[1
]}

机构：

[1] Univ Navarra, Fac Farm, Dept Farm & Tecnol Farmaceut, E-31080 Pamplona, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1031卷 / 1-2期

关键词：

rifampicin; antibiotics;

D O I：

10.1016/j.chroma.2003.12.041

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A HPLC-UV method has been developed for assaying rifampicin in plasma and liver. The assay involved a liquid-liquid extraction procedure with dichloromethane-pentane (1:1). An Ultrabase-C-18 column and a simple mobile phase consisting of a water (pH 2.27)-acetonitrile (40:60, v/v) mixture were used. The flow-rate was 1 ml/min and the effluent was monitored at 333 nm. Results from the HPLC analyses showed that the assay method is linear in the ranges 0.1-1 and 1-50 mug/ml for plasma, and 0.6-40 mug/g for liver. Intra- and inter-day R.S.D. were below 15% for all the sample types. Recoveries averaged 83 and 95% for plasma and liver, respectively. The method is being successfully applied to determine rifampicin in plasma and liver samples taken during pharmacokinetic studies in rats. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：289 / 294

页数：6

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