Separation of 100-kilobase DNA molecules in 10 seconds

被引：111

作者：

Bakajin, O

Duke, TAJ

Tegenfeldt, J

Chou, CF

Chan, SS

Austin, RH ^{[1
]}

Cox, EC

机构：

[1] Princeton Univ, Dept Phys, Princeton, NJ 08544 USA

[2] Cavendish Lab, Cambridge CB3 0HE, England

[3] Princeton Univ, Dept Biol Mol, Princeton, NJ 08544 USA

来源：

ANALYTICAL CHEMISTRY | 2001年 / 73卷 / 24期

关键词：

D O I：

10.1021/ac015527o

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Long double-stranded DNA molecules ware separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and lambda (48.5 kbp) DNAs could be resolved into two clearly separated bands in similar to 10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array.

引用

页码：6053 / 6056

页数：4

共 19 条

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