Wild bitter melon (Momordica charantia Linn. var. abbreviata Ser.) extract and its bioactive components suppress Propionibacterium acnes-induced inflammation

被引：62

作者：

Hsu, Chin ^{[1
]}

Tsai, Tsung-Hsien ^{[2
,3
]}

Li, You-Yi ^{[4
]}

Wu, Wen-Huey ^{[4
]}

Huang, Ching-Jang ^{[1
]}

Tsai, Po-Jung ^{[4
]}

机构：

[1] Natl Taiwan Univ, Coll Life Sci, Dept Biochem Sci & Technol, Taipei 10764, Taiwan

[2] Taipei Municipal Wan Fang Hosp, Dept Dermatol, Taipei, Taiwan

[3] Taipei Med Univ, Taipei, Taiwan

[4] Natl Taiwan Normal Univ, Dept Human Dev & Family Studies, Taipei, Taiwan

来源：

FOOD CHEMISTRY | 2012年 / 135卷 / 03期

关键词：

Wild bitter melon; Pro pionibacterium acnes; Anti-inflammation; Peroxisome proliferator-activated receptor; PROLIFERATOR-ACTIVATED RECEPTORS; PPAR-ALPHA; IN-VITRO; MATRIX METALLOPROTEINASES; CYTOKINE RESPONSES; HUMAN MONOCYTES; UP-REGULATION; HUMAN SKIN; KAPPA-B; EXPRESSION;

D O I：

10.1016/j.foodchem.2012.05.045

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

In this study, we aimed to evaluate the inhibitory effect of wild bitter melons (WBM: Momordica charantia Linn. var. abbreviata Ser.) on Propionibacterium acnes-induced inflammation and to identify the bioactive components. Our results showed that ethyl acetate (EA) extract of WBM fruit in vitro potently suppressed pro-inflammatory cytokine and matrix metalloproteinase (MMP)-9 levels in P. acnes-stimulated THP-1 cells. Furthermore, concomitant intradermal injection of WBM EA extract in mice effectively attenuated P. acnes-induced ear swelling and granulomatous inflammation. To further investigate the bioactive components, we found that both saponifiable (S) and nonsaponifiable (NS) fractions of WBM EA extract significantly suppressed pro-inflammatory cytokine and MMP-9 levels. Phytol and lutein, identified in the NS fraction, also inhibited cytokine production. Moreover, S and NS fractions of EA extract, phytol and lutein, activated peroxisome proliferator-activated receptor (PPAR) alpha and beta in the transactivation assay. Our results suggested that PPAR alpha or PPAR gamma signalling may contribute, at least in part, to the anti-inflammatory activity of WBM. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：976 / 984

页数：9

共 42 条

[1]

PPAR-γ Activation As an Anti-inflammatory Therapy for Respiratory Virus Infections [J].

Bassaganya-Riera, Josep ;

Song, Rong ;

Roberts, Paul C. ;

Hontecillas, Raquel .

VIRAL IMMUNOLOGY, 2010, 23 (04) :343-352

[2]

Regulation of inflammation by PPARs: a future approach to treat lung inflammatory diseases? [J].

Becker, Julien ;

Delayre-Orthez, Carine ;

Frossard, Nelly ;

Pons, Francoise .

FUNDAMENTAL & CLINICAL PHARMACOLOGY, 2006, 20 (05) :429-447

[3]

Antioxidant activity, lipid peroxidation and skin diseases. What's new [J].

Briganti, S ;

Picardo, M .

JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY, 2003, 17 (06) :663-669

[4]

In vivo and in vitro studies to identify the hypoglycaemic constituents of Momordica charantia wild variant WB24 [J].

Chang, Chi-I ;

Tseng, Hsin-I ;

Liao, Yun-Wen ;

Yen, Chia-Hung ;

Chen, Tz-Min ;

Lin, Chen-Chen ;

Cheng, Hsueh-Ling .

FOOD CHEMISTRY, 2011, 125 (02) :521-528

[5]

Bitter gourd (Momordica charantia) extract activates peroxisome proliferator-activated receptors and upregulates the expression of the acyl CoA oxidase gene in H4IIEC3 hepatoma cells [J].

Chao, CY ;

Huang, CJ .

JOURNAL OF BIOMEDICAL SCIENCE, 2003, 10 (06) :782-791

[6]

Resveratrol inhibits MMP-9 expression by up-regulating PPAR α expression in an oxygen glucose deprivation-exposed neuron model [J].

Cheng, Gang ;

Zhang, Xiang ;

Gao, Dakuan ;

Jiang, Xiaofan ;

Dong, Wenpeng .

NEUROSCIENCE LETTERS, 2009, 451 (02) :105-108

[7]

Isolation and identification of novel estrogenic compounds in yam tuber (Dioscorea alata cv. Tainung No. 2) [J].

Cheng, Wei-Yi ;

Ku, Yueh-Hsiung ;

Huang, Ching-Jang .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (18) :7350-7358

[8]

FREE-RADICAL SCAVENGING OF LUTEIN IN-VITRO [J].

CHOPRA, M ;

WILLSON, RL ;

THURNHAM, DI .

CAROTENOIDS IN HUMAN HEALTH, 1993, 691 :246-249

[9]

Fractionation and identification of 9c, 11t, 13t-conjugated linolenic acid as an activator of PPARα in bitter gourd (Momordica charantia L.) [J].

Chuang, Chia-Ying ;

Hsu, Chin ;

Chao, Che-Yi ;

Wein, Yung-Shung ;

Kuo, Yueh-Hsiung ;

Huang, Ching-jang .

JOURNAL OF BIOMEDICAL SCIENCE, 2006, 13 (06) :763-772

[10]

The role of the peroxisome proliferator-activated receptor-α (PPAR-α) in the regulation of acute inflammation [J].

Cuzzocrea, Salvatore ;

Mazzon, Emanuela ;

Di Paola, Rosanna ;

Peli, Angelo ;

Bonato, Andrea ;

Britti, Domenico ;

Genovese, Tiziana ;

Muia, Carmelo ;

Crisafulli, Concetta ;

Caputi, Achille P. .

JOURNAL OF LEUKOCYTE BIOLOGY, 2006, 79 (05) :999-1010

← 1 2 3 4 5 →