Role of menaquinones in Fe(III) reduction by membrane fractions of Shewanella putrefaciens

被引:38
作者
Saffarini, DA
Blumerman, SL
Mansoorabadi, KJ
机构
[1] Univ Wisconsin, Dept Biol Sci, Milwaukee, WI 53211 USA
[2] Univ Massachusetts, Amherst, MA 01003 USA
关键词
D O I
10.1128/JB.184.3.846-848.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Two Tn5-generated mutants of Shewanella putrefaciens with insertions in menD and menB were isolated and analyzed. Both mutants were deficient in the use of several terminal electron acceptors, including Fe(III). This deficiency was overcome by the addition of menaquinone (vitamin K-2). Isolated membrane fractions from both mutants were unable to reduce Fe(III) in the absence of added menaquinone when formate was used as the electron donor. These results indicate that menaquinones are essential components for the reduction of Fe(III) by both whole cells and purified membrane fractions when formate or lactate is used as the electron donor.
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页码:846 / 848
页数:3
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