Immunohistochemical localization of transforming growth factor beta 1 and beta 2 in the fetal and neonatal rat ovary

The localization of transforming growth factor beta (TGF beta) in the differentiating ovary (from fetal day 13.5 to postnatal day 14) was investigated immunohistochemically using polyclonal antibodies for TGF beta 1 and TGF beta 2. Immunostaining was undetectable in the gonadal primordium on fetal day 13.5. From fetal day 14.5 and throughout fetal life, there was intense immunostaining for TGF beta 1 and faint staining for TGF beta 2 in some ovarian somatic cells which were identified as epithelial cells at the end of fetal life. This pattern of staining was also found in the presumptive granulosa cells present between the oocytes on postnatal day 1. The staining for TGF beta 2 persisted while the staining for TGF beta 1 decreased in the granulosa cells of primordial and primary follicles of older rats. In the subsequent stages of follicular development, the staining for TGF beta 1 disappeared while faint staining for TGF beta 2 persisted in the granulosa cells of secondary and small antral (postnatal day 14) follicles. On day 14, the newly functional thecal/interstitial cells were moderately stained for TGF beta 2 and intensely stained for TGF beta 1. These results plus our previous immunolocalization of TGF beta 1 in the fetal testis [13] show that, in both sexes: (1) TGF beta 1 is detectable in the gonads on the same fetal age (day 14.5); (2) TGF beta 1 is present in the somatic cells which are the precursors of Sertoli and granulosa cells, at the time of their organization in seminiferous tubules or in primordial follicles; and (3) TGF beta 1 is largely present in cells synthesizing androgens, from the onset of their steroidogenic capacity (fetal day 16.5 for Leydig cells and postnatal day 14 for thecal/interstitial cells).