Poliovirus induces apoptosis in the mouse central nervous system

被引:82
作者
Girard, S
Couderc, T
Destombes, J
Thiesson, D
Delpeyroux, F
Blondel, B
机构
[1] Inst Pasteur, Unite Neurovirol & Regenerat Syst Nerveux, F-75724 Paris 15, France
[2] Inst Pasteur, Lab Epidemiol Mol Enterovirus, F-75724 Paris, France
[3] Fac Med Pitie Salpetriere, Lab Cytosquelette & Dev, CNRS, URA 2115, F-75634 Paris 13, France
关键词
D O I
10.1128/JVI.73.7.6066-6072.1999
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Poliovirus (PV) is the etiological agent of human paralytic poliomyelitis. Paralysis results from the destruction of motoneurons, a consequence of PV replication. However, the PV-induced process leading to the death of motoneurons is not well known. We investigated whether PV-induced central nervous system (CNS) injury is associated with apoptosis by using mice as animal models. Transgenic mice expressing the human PV receptor were infected intracerebrally with either the neurovirulent PV-1 Mahoney strain or a paralytogenic dose of the attenuated PV-1 Sabin strain, Nontransgenic mice were infected with a mouse-adapted PV-1 Mahoney mutant. DNA fragmentation was demonstrated in CNS tissue from paralyzed mice by visualization of DNA oligonucleosomal laddering and by enzyme-linked immunosorbent assay. Viral antigens and DNA fragmentation detected by the in situ terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end-labeling technique were colocalized in neurons of spinal cords from paralyzed mice. In addition, morphological changes characteristic of cells undergoing apoptosis were observed in motoneurons by electron microscopy. Thus, we show that PV multiplication and CNS injury during paralytic poliomyelitis are associated with apoptosis.
引用
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页码:6066 / 6072
页数:7
相关论文
共 35 条
  • [1] Upregulation of fas ligand expression by human immunodeficiency virus in human macrophages mediates apoptosis of uninfected T lymphocytes
    Badley, AD
    McElhinny, JA
    Leibson, PJ
    Lynch, DH
    Alderson, MR
    Paya, CV
    [J]. JOURNAL OF VIROLOGY, 1996, 70 (01) : 199 - 206
  • [2] CELL-DEATH IN HEALTH AND DISEASE - THE BIOLOGY AND REGULATION OF APOPTOSIS
    BELLAMY, COC
    MALCOMSON, RDG
    HARRISON, DJ
    WYLLIE, AH
    [J]. SEMINARS IN CANCER BIOLOGY, 1995, 6 (01) : 3 - 16
  • [3] DETECTION BY MONOCLONAL-ANTIBODIES OF AN ANTIGENIC DETERMINANT CRITICAL FOR POLIOVIRUS NEUTRALIZATION PRESENT ON VP1 AND ON HEAT-INACTIVATED VIRIONS
    BLONDEL, B
    AKACEM, O
    CRAINIC, R
    COUILLIN, P
    HORODNICEANU, F
    [J]. VIROLOGY, 1983, 126 (02) : 707 - 710
  • [4] HISTOPATHOLOGIC BASIS OF CLINICAL FINDINGS IN POLIOMYELITIS
    BODIAN, D
    [J]. AMERICAN JOURNAL OF MEDICINE, 1949, 6 (05) : 563 - 578
  • [5] A cytopathogenic, apoptosis-inducing variant of hepatitis A virus
    Brack, K
    Frings, W
    Dotzauer, A
    Vallbracht, A
    [J]. JOURNAL OF VIROLOGY, 1998, 72 (04) : 3370 - 3376
  • [6] Caspase activation and specific cleavage of substrates after coxsackievirus B3-induced cytopathic effect in HeLa cells
    Carthy, CM
    Granville, DJ
    Watson, KA
    Anderson, DR
    Wilson, JE
    Yang, DC
    Hunt, DWC
    McManus, BM
    [J]. JOURNAL OF VIROLOGY, 1998, 72 (09) : 7669 - 7675
  • [7] MOLECULAR CHARACTERIZATION OF MOUSE-VIRULENT POLIOVIRUS TYPE-1 MAHONEY MUTANTS - INVOLVEMENT OF RESIDUES OF POLYPEPTIDES VP1 AND VP2 LOCATED ON THE INNER SURFACE OF THE CAPSID PROTEIN SHELL
    COUDERC, T
    HOGLE, J
    LEBLAY, H
    HORAUD, F
    BLONDEL, B
    [J]. JOURNAL OF VIROLOGY, 1993, 67 (07) : 3808 - 3817
  • [8] Mouse adaptation determinants of poliovirus type 1 enhance viral uncoating
    Couderc, T
    Delpeyroux, F
    LeBlay, H
    Blondel, B
    [J]. JOURNAL OF VIROLOGY, 1996, 70 (01) : 305 - 312
  • [9] Despres P, 1998, J VIROL, V72, P823
  • [10] Persistent poliovirus infection in mouse motoneurons
    Destombes, J
    Couderc, T
    Thiesson, D
    Girard, S
    Wilt, SG
    Blondel, B
    [J]. JOURNAL OF VIROLOGY, 1997, 71 (02) : 1621 - 1628