A dimeric mutant of human pancreatic ribonuclease with selective cytotoxicity toward malignant cells

被引:64
作者
Piccoli, R
Di Gaetano, S
De Lorenzo, C
Grauso, M
Monaco, C
Spalletti-Cernia, D
Laccetti, P
Cinátl, J
Matousek, J
D'Alessio, G
机构
[1] Univ Naples, Dipartimento Biol & Patol Cellulare & Mol L Calif, CNR, Ctr Endocrinol & Oncol Sperimentale, I-80131 Naples, Italy
[2] KlinLab, Prague 16920, Czech Republic
[3] Acad Sci Czech Republ, Inst Anim Physiol & Genet, Dept Genet, CR-27721 Libechov, Czech Republic
[4] Univ Frankfurt, Dept Haematol & Oncol, D-6000 Frankfurt, Germany
关键词
D O I
10.1073/pnas.96.14.7768
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Monomeric human pancreatic RNase, devoid of any biological activity other than its RNA degrading ability, was engineered into a dimeric protein with a cytotoxic action on mouse and human tumor cells, but lacking any appreciable toxicity on mouse and human normal cells. This dimeric variant of human pancreas RNase selectively sensitizes to apoptotic death cells derived from a human thyroid tumor. Because of its selectivity for tumor cells, and because of its human origin, this protein represents a potentially very attractive, novel tool for anticancer therapy.
引用
收藏
页码:7768 / 7773
页数:6
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