Method for quantification of morphine and its 3-and 6-glucuronides, codeine, codeine glucuronide and 6-monoacetylmorphine in human blood by liquid chromatography-electrospray mass spectrometry for routine analysis in forensic toxicology

被引：50

作者：

Dienes-Nagy, A ^{[1
]}

Rivier, L ^{[1
]}

Giroud, C ^{[1
]}

Augsburger, M ^{[1
]}

Mangin, P ^{[1
]}

机构：

[1] Inst Univ Med Legale, CH-1005 Lausanne, Switzerland

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 854卷 / 1-2期

关键词：

forensic analysis; morphine; codeine; glucuronides; monoacetylmorphine;

D O I：

10.1016/S0021-9673(99)00750-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Simultaneous determination of opiates and their glucuronides in body fluids has a great practical interest in the forensic assessment of heroin intoxication. A selective and sensitive method for quantification of morphine and its 3- and 6-glucuronides, codeine, codeine glucuronide and 6-monoacetylmorphine (6-MAM) based on liquid chromatography-electrospray ionisation mass spectrometry is described. The drugs were analysed in human autopsy whole blood after solid-phase extraction on a C-8 cartridge. The separation was performed on an ODS column in acetonitrile (analysis time 15 min). For the quantitative analysis, deuterated analogues of each compound were used as internal standards. Selected-ion monitoring was applied where the molecular ion was chosen for quantification. The limits of quantification were 0.5 ng/ml for morphine and B-MAM and 1 ng/ml for the 6-glucuronide of morphine, codeine-6-glucuronide and codeine and 5 ng/ml for the 3-glucuronide of morphine. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：109 / 118

页数：10

共 19 条

[1] MORPHINE AND MORPHINE GLUCURONIDES IN SERUM OF HEROIN CONSUMERS AND IN HEROIN-RELATED DEATHS DETERMINED BY HPLC WITH NATIVE FLUORESCENCE DETECTION [J].