Expression and functional role of bTRPC1 channels in native endothelial cells

被引：68

作者：

Antoniotti, S

Lovisolo, D

Pla, AF

Munaron, L

机构：

[1] Univ Turin, Dept Anim & Human Biol, I-10123 Turin, Italy

[2] Udr Torino Univ, INFM, Turin, Italy

来源：

FEBS LETTERS | 2002年 / 510卷 / 03期

关键词：

transient receptor potential-C1; basic fibroblast growth factor; calcium influx; endothelial cells;

D O I：

10.1016/S0014-5793(01)03256-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have analyzed the expression and localization of bovine transient receptor potential-C1 (bTRPC1) in bovine aortic endothelial cells, and its possible involvement in the store-independent calcium influx induced by basic fibroblast growth factor (bFGF). RT-PCR experiments confirmed the existence of two btrpe1 mRNA isoforms; conversely, the btrpc3 gene was not transcribed. Anti-TRPC1 antibody revealed the presence of the protein in the membrane-rich compartment only. Application of anti-TRPC1 during the response to bFGF caused a partial but significant reduction of calcium entry. This is the first evidence of TRP channel involvement in a non-capacitative calcium influx induced by a biologically relevant agonist such as the angiogenic factor bFGF in native endothelial cells. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：189 / 195

页数：7

共 39 条

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