Determination of astragaloside IV in Radix astragali (Astragalus membranaceus var. monghulicus) using high-performance liquid chromatography with evaporative light-scattering detection

被引：25

作者：

Li, WK ^{[1
]}

Fitzloff, JF ^{[1
]}

机构：

[1] Univ Illinois, Coll Pharm, Dept Med Chem & Pharmacognosy, Program Collaborat Res Pharmaceut Sci, Chicago, IL 60612 USA

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2001年 / 39卷 / 11期

关键词：

D O I：

10.1093/chromsci/39.11.459

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reverse-phase high-performance liquid chromatographic method is developed for the determination of astragaloside IV, a characteristic constituent in Radix Astragali. Samples are analyzed by means of a reverse-phase column (Zorbax Eclipse XDB C18) using acetonitrile and water under gradient conditions as the mobile phase for 30 min. An evaporative light-scattering detector is used and set at an evaporating temperature of 43°C with a nebulizing gas (compressed air) pressure of 3.4 bar. The detection limit (signal-to-noise ratio > 5) of astragaloside IV is 40 ng on-column.

引用

页码：459 / 462

页数：4

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