Differential DNA binding activities of the transcription factors AP-1 and Oct-1 during light-induced apoptosis of photoreceptors

The activity of transcription factors like AP-1 and Oct-1 is critical for the regulation of gene expression. Whereas Oct-1 mainly regulates the expression of housekeeping genes, AP-1 is often involved in cellular responses to external stimuli and plays an essential role in the regulation of light-induced apoptosis of mouse retinal photoreceptors. In this study, we investigated AP-I and Oct-1 DNA binding activity and AP-1 complex composition in the mouse retina during light-induced photoreceptor apoptosis. AP-1 DNA binding activity was: low in dark-adapted animals but was transiently elevated within 12 h after exposure of mice to apoptosis-inducing levels of white fluorescent light. Maximal AP-1 activity was found 6 h after light-exposure. Antibody interference analysis at 6 h after damaging light exposure and under normal light conditions revealed that the major fraction of AP-1 consists of c-Fos/JunD heterodimers in both situations. In contrast to AP-1, Oct-1 DNA binding activity was maximal in dark-adapted animals and was reduced during photoreceptor apoptosis. Transient induction of AP-1 (c-Fos/JunD) and inactivation of Oct-1 may be crucial events for light-mediated apoptosis of retinal photoreceptors. (C) 1999 Elsevier Science Ltd. All rights reserved.