Genomic and genetic analysis of Myb-related genes that regulate anthocyanin biosynthesis in grape berry skin

被引：174

作者：

Azuma, Akifumi ^{[1
]}

Kobayashi, Shozo ^{[1
]}

Mitani, Nobuhito ^{[1
]}

Shiraishi, Mikio ^{[2
]}

Yamada, Masahiko ^{[1
]}

Ueno, Toshihito ^{[1
]}

Kono, Atsushi ^{[1
]}

Yakushiji, Hiroshi ^{[1
]}

Koshita, Yoshiko ^{[1
]}

机构：

[1] Natl Inst Fruit Tree Sci, Grape & Persimmon Res Stn, Hiroshima 7392494, Japan

[2] Fukuoka Agr Res Ctr, Fukuoka 8188549, Japan

来源：

THEORETICAL AND APPLIED GENETICS | 2008年 / 117卷 / 06期

关键词：

D O I：

10.1007/s00122-008-0840-1

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

As a result of natural hybridization and human selection over millennia, the skin colors of grapes have become greatly diversified. The color is determined by the quantity and composition of anthocyanins. Color-skinned cultivars accumulate anthocyanins in their skins, whereas white-skinned cultivars do not. Myb-related transcription-factor genes such as VvmybA1 regulate anthocyanin biosynthesis. VvMYBA2r, VlmybA1-1, VlmybA1-2, and VlmybA2, which are homologs of VvmybA1, also regulate anthocyanin biosynthesis. In this study, we isolated a novel Myb-related sequence, VlmybA1-3, from cultivars of Vitis labruscana (Vitis vinifera x Vitis labrusca) by means of inverse PCR, and confirmed by means of transient gene expression assay that the gene regulates anthocyanin biosynthesis in grape berry skin. Seedlings of V. labruscana with two functional haplotypes at a region of berry color loci accumulated more anthocyanins than seedlings with a single functional haplotype. In addition, we investigated the haplotypes at the region in 35 cultivars (both V. vinifera and V. labruscana), and found certain typical characteristics. These findings will contribute to the selection of seedlings with high anthocyanin quantities in breeding programs for wine and table grapes, and will help elucidate the origin and evolution of Vitis species.

引用

页码：1009 / 1019

页数：11

共 39 条

[1]

Azuma A, 2007, VITIS, V46, P154

[2] The A locus that controls anthocyanin accumulation in pepper encodes a MYB transcription factor homologous to Anthocyanin2 of Petunia [J].

Borovsky, Y ;

Oren-Shamir, M ;

Ovadia, R ;

De Jong, W ;

Paran, I .

THEORETICAL AND APPLIED GENETICS, 2004, 109 (01) :23-29

[3]

Boss P. K., 1996, Australian Journal of Grape and Wine Research, V2, P163, DOI 10.1111/j.1755-0238.1996.tb00104.x

[4] Expression of anthocyanin biosynthesis pathway genes in red and white grapes [J].

Boss, PK ;

Davies, C ;

Robinson, SP .

PLANT MOLECULAR BIOLOGY, 1996, 32 (03) :565-569

[5] Water deficits accelerate ripening and induce changes in gene expression regulating flavonoid biosynthesis in grape berries [J].

Castellarin, Simone D. ;

Matthews, Mark A. ;

Di Gaspero, Gabriele ;

Gambetta, Gregory A. .

PLANTA, 2007, 227 (01) :101-112

[6] Genetic mapping of grapevine (Vitis vinifera L.) applied to the detection of QTLs for seedlessness and berry weight [J].

Doligez, A ;

Bouquet, A ;

Danglot, Y ;

Lahogue, F ;

Riaz, S ;

Meredith, CP ;

Edwards, KJ ;

This, P .

THEORETICAL AND APPLIED GENETICS, 2002, 105 (05) :780-795

[7] Quantitative trait locus analysis of fungal disease resistance factors on a molecular map of grapevine [J].

Fischer, BM ;

Salakhutdinov, I ;

Akkurt, M ;

Eibach, R ;

Edwards, KJ ;

Töpfer, R ;

Zyprian, EM .

THEORETICAL AND APPLIED GENETICS, 2004, 108 (03) :501-515

[8] Anthocyanin production by over-expression of grape transcription factor gene VlmybA2 in transgenic tobacco and Arabidopsis [J].

Geekiyanage, Sudarshanee ;

Takase, Tomoyuki ;

Ogura, Yasunobu ;

Kiyosue, Tomohiro .

PLANT BIOTECHNOLOGY REPORTS, 2007, 1 (01) :11-18

[9]

Goto-Yamamoto N, 2006, AM J ENOL VITICULT, V57, P105

[10] ABA and sugar effects on anthocyanin formation in grape berry cultured in vitro [J].

Hiratsuka, S ;

Onodera, H ;

Kawai, Y ;

Kubo, T ;

Itoh, H ;

Wada, R .

SCIENTIA HORTICULTURAE, 2001, 90 (1-2) :121-130

← 1 2 3 4 →