Optical algal biosensor using alkaline phosphatase for determination of heavy metals

被引：74

作者：

Durrieu, C

Tran-Minh, C

机构：

[1] Ecole Natl Super Mines, Ctr SPIN Genie Enzymat, F-42023 St Etienne Du Rouvray 2, France

[2] Ecole Natl Travaux Publ Etat, Lab Sci Environm, F-69518 Vaulx En Velin, France

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2002年 / 51卷 / 03期

关键词：

biosensor; algae; Chlorella; alkaline phosphatase; fluorescence; heavy metals; ecotoxicology;

D O I：

10.1006/eesa.2001.2140

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A biosensor is constructed to detect heavy metals from inhibition of alkaline phosphatase (AP) present on the external membrane of Chlorella vulgaris microalgae. The microalgal cells are immobilized on removable membranes placed in front of the tip of an optical fiber bundle inside a homemade microcell. C vulgaris was cultivated in the laboratory and its alkaline phosphatase activity is strongly inhibited in the presence of heavy metals. This property has been used for the determination of those toxic compounds. (C) 2002 Elsevier Science (USA).

引用

页码：206 / 209

页数：4

共 12 条

[1] ALBERY WJ, 1990, BIOSENS BIOELECTRON, V5, P379
[2] THE DETECTION AND IDENTIFICATION OF METAL AND ORGANIC POLLUTANTS IN POTABLE WATER USING ENZYME ASSAYS SUITABLE FOR SENSOR DEVELOPMENT
COWELL, DC
DOWMAN, AA
ASHCROFT, T
CAFFOOR, I
[J]. BIOSENSORS & BIOELECTRONICS, 1995, 10 (6-7) : 509 - 516
[3] DETECTION OF HEAVY-METAL SALTS WITH BIOSENSORS BUILT WITH AN OXYGEN-ELECTRODE COUPLED TO VARIOUS IMMOBILIZED OXIDASES AND DEHYDROGENASES
GAYET, JC
HAOUZ, A
GELOSOMEYER, A
BURSTEIN, C
[J]. BIOSENSORS & BIOELECTRONICS, 1993, 8 (3-4) : 177 - 183
[4] A two-stage minibioreactor system for continuous toxicity monitoring
Gu, MB
Gil, GC
Kim, JH
[J]. BIOSENSORS & BIOELECTRONICS, 1999, 14 (04) : 355 - 361
[5] *INT ORG STAND, 1989, 8692 ISO
[6] Fiber optic biosensor using Chlorella vulgaris for determination of toxic compounds
Naessens, M
Leclerc, JC
Tran-Minh, C
[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2000, 46 (02) : 181 - 185
[7] BIOSENSORS BASED ON CELL AND TISSUE MATERIAL
RECHNITZ, GA
[J]. JOURNAL OF BIOTECHNOLOGY, 1990, 15 (03) : 201 - 217
[8] ROGERS KM, 1999, ENV SCI TECHNOL NEWS, P498
[9] USE OF ALGAL FLUORESCENCE FOR DETERMINATION OF PHYTOTOXICITY OF HEAVY-METALS AND PESTICIDES AS ENVIRONMENTAL-POLLUTANTS
SAMSON, G
POPOVIC, R
[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 1988, 16 (03) : 272 - 278
[10] An ISFET-algal (Chlamydomonas) hybrid provides a system for eco-toxicological tests
Schubnell, D
Lehmann, M
Baumann, W
Rott, FG
Wolf, B
Beck, CF
[J]. BIOSENSORS & BIOELECTRONICS, 1999, 14 (05) : 465 - 472

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