Inducible RNA Interference of brlAβ in Aspergillus nidulans

被引：18

作者：

Barton, L. M. ^{[1
]}

Prade, R. A. ^{[1
]}

机构：

[1] Oklahoma State Univ, Dept Microbiol & Mol Genet, Stillwater, OK 74078 USA

来源：

EUKARYOTIC CELL | 2008年 / 7卷 / 11期

关键词：

D O I：

10.1128/EC.00142-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An inducible RNA interference (RNAi) construct composed of inverted repeating alcA promoters flanking the developmental regulatory gene brlA beta was tested in Aspergillus nidulans. On inducing medium, the RNAi strains failed to sporulate and lacked brlA alpha and brlA beta expression. RNAi was specific for brlA beta, but not brlA alpha, silencing, indicating brlA alpha regulation by brlA beta.

引用

页码：2004 / 2007

页数：4

共 26 条

[1] UPSTREAM ELEMENTS REPRESS PREMATURE EXPRESSION OF AN ASPERGILLUS DEVELOPMENTAL REGULATORY GENE [J].

ADAMS, TH ;

TIMBERLAKE, WE .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (09) :4912-4919

[2] BRLA IS NECESSARY AND SUFFICIENT TO DIRECT CONIDIOPHORE DEVELOPMENT IN ASPERGILLUS-NIDULANS [J].

ADAMS, TH ;

BOYLAN, MT ;

TIMBERLAKE, WE .

CELL, 1988, 54 (03) :353-362

[3] Asexual sporulation in Aspergillus nidulans [J].

Adams, TH ;

Wieser, JK ;

Yu, JH .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (01) :35-+

[4] Gene silencing in Caenorhabditis elegans by transitive RNA interference [J].

Alder, MN ;

Dames, S ;

Gaudet, J ;

Mango, SE .

RNA, 2003, 9 (01) :25-32

[5] KINETICS OF DIFFERENTIATION OF CONIDIOPHORES AND CONIDIA BY COLONIES OF ASPERGILLUS-NIDULANS [J].

AXELROD, DE .

JOURNAL OF GENERAL MICROBIOLOGY, 1972, 73 (NOV) :181-&

[6] ISOLATION AND PHYSICAL CHARACTERIZATION OF 3 ESSENTIAL CONIDIATION GENES FROM ASPERGILLUS-NIDULANS [J].

BOYLAN, MT ;

MIRABITO, PM ;

WILLETT, CE ;

ZIMMERMAN, CR ;

TIMBERLAKE, WE .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (09) :3113-3118

[7] Involvement of small RNAs and role of the qde genes in the gene silencing pathway in Neurospora [J].

Catalanotto, C ;

Azzalin, G ;

Macino, G ;

Cogoni, C .

GENES & DEVELOPMENT, 2002, 16 (07) :790-795

[8]

CHANG YC, 1993, GENETICS, V133, P29

[9]

CLUTTERBUCK AJ, 1969, GENETICS, V63, P317

[10] RNA silencing gene truncation in the filamentous fungus Aspergillus nidulans [J].

Hammond, T. M. ;

Bok, J. W. ;

Andrewski, M. D. ;

Reyes-Dominguez, Y. ;

Scazzocchio, C. ;

Keller, N. P. .

EUKARYOTIC CELL, 2008, 7 (02) :339-349

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