Species Identification of Campylobacter jejuni ssp jejuni and C. coli by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry and PCR

被引：23

作者：

Kolinska, R. ^{[1
]}

Drevinek, M. ^{[2
]}

Jakubu, V. ^{[1
]}

Zemlickova, H. ^{[1
]}

机构：

[1] Natl Inst Publ Hlth, Prague 10042, Czech Republic

[2] Natl Inst Nucl Biol & Chem Protect, Pribram Kamenna, Czech Republic

来源：

FOLIA MICROBIOLOGICA | 2008年 / 53卷 / 05期

关键词：

D O I：

10.1007/s12223-008-0061-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Campylobacter species strains (n = 42; isolated from clinical samples and deposited in Czech National Collection of Type Cultures, Prague) originally phenotypically (and biochemically) identified as Campylobacter jejuni were re-classified using molecular biological and mass spectrometric methods. Whole-cell MALDI-TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry) separated the isolates into two genetically related strains - C. jejuni (n = 26) and C. coli (n = 16) and, moreover, distinguished the intimate details in the group of tested strains. It also made it possible to create the MALDI-TOF MS dendrogram; similarly, the spectral characteristics were used for the 3D cluster analysis. Polymerase chain reaction (PCR) confirmed the results obtained by mass spectrometry. Both methods (PCR and MALDI-TOF MS) gave the same results which supports their suitability in the rapid and accurate Campylobacter-species determination.

引用

页码：403 / 409

页数：7

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[1] Detection and identification of bacterial enteropathogens by polymerase chain reaction and conventional techniques in childhood acute gastroenteritis in Gaza, Palestine [J].