Specific serology for emerging human coronaviruses by protein microarray

被引：64

作者：

Reusken, C. ^{[1
]}

Mou, H. ^{[2
]}

Godeke, G. J. ^{[1
]}

van der Hoek, L. ^{[3
]}

Meyer, B. ^{[4
]}

Mueller, M. A. ^{[4
]}

Haagmans, B. ^{[5
]}

de Sousa, R. ^{[1
]}

Schuurman, N. ^{[2
]}

Dittmer, U. ^{[6
]}

Rottier, P. ^{[2
]}

Osterhaus, A. ^{[5
]}

Drosten, C. ^{[4
]}

Bosch, B. J. ^{[2
]}

Koopmans, M. ^{[1
,5
]}

机构：

[1] Natl Inst Publ Hlth & Environm, Div Virol, Ctr Infect Dis Control, NL-3720 BA Bilthoven, Netherlands

[2] Univ Utrecht, Fac Vet Med, Dept Immunol & Infect Dis, Utrecht, Netherlands

[3] Univ Amsterdam, Acad Med Ctr, Ctr Infect & Immun Amsterdam, Dept Med Microbiol,Lab Expt Virol, NL-1105 AZ Amsterdam, Netherlands

[4] Univ Bonn, Med Ctr, Inst Virol, Bonn, Germany

[5] Erasmus MC, Dept Virosci, Rotterdam, Netherlands

[6] Univ Duisburg Essen, Univ Hosp Essen, Inst Virol, Essen, Germany

来源：

EUROSURVEILLANCE | 2013年 / 18卷 / 14期

关键词：

INFECTION; VIRUSES; NL63;

D O I：

10.2807/1560-7917.ES2013.18.14.20441

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We present a serological assay for the specific detection of IgM and IgG antibodies against the emerging human coronavirus hCoV-EMC and the SARS-CoV based on protein microarray technology. The assay uses the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens. The assay has been validated extensively using putative cross-reacting sera of patient cohorts exposed to the four common hCoVs and sera from convalescent patients infected with hCoV-EMC or SARS-CoV.

引用

页码：2 / 8

页数：7

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