Speciation of selenoamino acids and organoselenium compounds in selenium-enriched yeast using high-performance liquid chromatography inductively coupled plasma mass spectrometry

被引：137

作者：

Bird, SM

Uden, PC

Tyson, JF

Block, E

Denoyer, E

机构：

[1] UNIV MASSACHUSETTS,DEPT CHEM,AMHERST,MA 01003

[2] SUNY ALBANY,DEPT CHEM,ALBANY,NY 12222

[3] PERKIN ELMER CORP,NORWALK,CT 06859

来源：

JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY | 1997年 / 12卷 / 07期

关键词：

selenoamino acids; selenium; speciation; high-performance liquid chromatography; inductively coupled plasma mass spectrometry; ion pair; selenium-enriched yeast;

D O I：

10.1039/a701429e

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

As part of an ongoing study to identify selenium compounds with cancer chemopreventive activity, selenium-enriched yeast was analyzed by HPLC-ICP-MS. More than twenty selenium-containing species were found in hot water and enzymatic hydrolysis extracts of the yeast, Trifluoroacetic acid was used as an ion-pairing agent in a water-methanol mobile phase with reversed-phase chromatography on an octylsilane stationary phase. The presence of selenocystine, selenomethionine and methylselenocysteine was confirmed by comparative retention of standards. The column efficiency was 8500 theoretical plates and the mobile-phase was compatible with standard ICP-MS operating conditions.

引用

页码：785 / 788

页数：4

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