Cibacron blue F3G-A attached poly(vinyl alcohol) particles for specific albumin adsorption

被引：43

作者：

Denizli, A ^{[1
]}

Tuncel, A ^{[1
]}

Kozluca, A ^{[1
]}

Ecevit, K ^{[1
]}

Piskin, E ^{[1
]}

机构：

[1] HACETTEPE UNIV,DEPT CHEM ENGN,TR-06420 ANKARA,TURKEY

来源：

SEPARATION SCIENCE AND TECHNOLOGY | 1997年 / 32卷 / 05期

关键词：

D O I：

10.1080/01496399708000942

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Poly(vinyl alcohol) (PVAL) particles (average size: 50 mu m) were prepared in the present study by chemical crosslinking of PVAL with glutaraldehyde in an organic dispersion oil phase. Cibacron Blue F3G-A was attached to these hydrophilic PVAL particles. Bovine serum albumin (BSA) adsorption onto these dye-attached PVAL particles from aqueous solutions containing different amounts of BSA in three different salts (i.e., NaCl, CaCl2, and NaSCN) at different pH and ionic strengths was investigated in batch reactors. The maximum adsorption capacity (about 35 mg BSA/g dye-attached PVAL) was observed around pH 6.0 in a medium containing NaCl with an ionic strength of 0.01. Nonspecific BSA adsorption on plain PVAL particles was almost zero. About 90% of the adsorbed BSA was desorbed by using a 0.5 M NaSCN desorption medium for 1 hour.

引用

页码：1003 / 1015

页数：13

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