Distribution of ClC-2 chloride channel in rat and human epithelial tissues

被引：107

作者：

Lipecka, J

Bali, M

Thomas, A

Fanen, P

Edelman, A ^{[1
]}

Fritsch, J

机构：

[1] Fac Med Necker Enfants Malad, INSERM, U467, F-75015 Paris, France

[2] Inst Fed Rech Xavier Bichat, INSERM, U410, F-75018 Paris, France

[3] Hop Henri Mondor, INSERM, U468, F-94010 Creteil, France

[4] Hop St Vincent de Paul, CNRS, Unite Propre Rech 1524, F-75674 Paris, France

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 2002年 / 282卷 / 04期

关键词：

small intestine; colon; ciliated cells; immunohistochemistry; antibody;

D O I：

10.1152/ajpcell.00291.2001

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The ubiquitous ClC-2 Cl- channel is thought to contribute to epithelial Cl- secretion, but the distribution of the ClC-2 protein in human epithelia has not been investigated. We have studied the distribution of ClC-2 in adult human and rat intestine and airways by immunoblotting and confocal microscopy. In the rat, ClC-2 was present in the lateral membranes of villus enterocytes and was predominant at the basolateral membranes of luminal colon enterocytes. The expression pattern of ClC-2 in the human intestine differed significantly, because ClC-2 was mainly detected in a supranuclear compartment of colon cells. We found significant expression of ClC-2 at the apex of ciliated cells in both rat and human airways. These results show that the distribution of ClC-2 in airways is consistent with participation of ClC-2 channels in Cl- secretion and indicate that extrapolation of results from studies of ClC-2 function in rat intestine to human intestine is not straightforward.

引用

页码：C805 / C816

页数：12

共 59 条

[1] CHLORIDE CHANNELS IN THE APICAL MEMBRANE OF NORMAL AND CYSTIC-FIBROSIS AIRWAY AND INTESTINAL EPITHELIA [J].