Quantitative analysis of cyclooxygenase-2 gene expression on acute gastric injury induced by ischemia-reperfusion in rats

被引：36

作者：

Kishimoto, Y ^{[1
]}

Wada, K ^{[1
]}

Nakamoto, K ^{[1
]}

Ashida, K ^{[1
]}

Kamisaki, Y ^{[1
]}

Kawasaki, H ^{[1
]}

Itoh, T ^{[1
]}

机构：

[1] TOTTORI UNIV, FAC MED, DEPT INTERNAL MED 2, YONAGO, TOTTORI 683, JAPAN

来源：

LIFE SCIENCES | 1997年 / 60卷 / 08期

关键词：

cyclooxygenase-2; reverse transcription competitive polymerase chain reaction ischemia-reperfusion; gastric injury; rat;

D O I：

10.1016/S0024-3205(96)00694-7

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The rat model of acute gastric damage induced by ischemia-reperfusion (I-R) has been used to evaluate the protective effect of various drugs on gastric injury. However, the quantitative expression state of cyclooxygenase-2 (COX-2), a protein which induces cytoprotective prostaglandins during inflammation, is still unknown in acute gastric injuriy induced by I-R. Therefore, we have quantitatively investigated the level of expression of COX-2 mRNA in injured gastric tissue of this model using the reverse transcription-competitive polymerase chain reaction method. The mRNA for COX-2 was expressed at low or undetectable levels in the normal gastric tissues in control rats, which were fasted for 18 hrs without I-R. The mRNA levels of COX-2 in injured gastric tissues were higher than those of control tissues between 6 hrs and 48 hrs after I-R. The maximum expression of COX-2 mRNA was recorded at 24 hrs (approximately a 200-foId increase). The expression state of COX-2, which has been ascertained in this study, should be useful in evaluating the effect of various drugs on the expression of COX-2 in acute gastric damage.

引用

页码：PL127 / PL133

页数：7

共 20 条

[1]

BAKER R, 1977, BRIT J SURG, V64, P24

[2] PRIMARY STRUCTURE OF PROSTAGLANDIN-G/H SYNTHASE FROM SHEEP VESICULAR GLAND DETERMINED FROM THE COMPLEMENTARY-DNA SEQUENCE [J].

DEWITT, DL ;

SMITH, WL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (05) :1412-1416

[3] PROSTAGLANDIN ENDOPEROXIDE SYNTHASE - REGULATION OF ENZYME EXPRESSION [J].

DEWITT, DL .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1083 (02) :121-134

[4] REGULATION OF EICOSANOID PRODUCTION AND MITOGENESIS IN RAT INTESTINAL EPITHELIAL-CELLS BY TRANSFORMING GROWTH-FACTOR-ALPHA, AND PHORBOL ESTER [J].

DUBOIS, RN ;

AWAD, J ;

MORROW, J ;

ROBERTS, LJ ;

BISHOP, PR .

JOURNAL OF CLINICAL INVESTIGATION, 1994, 93 (02) :493-498

[5]

FLETCHER BS, 1992, J BIOL CHEM, V267, P4338

[6]

FUNK CD, 1991, J BIOL CHEM, V266, P12508

[7] HUMAN PLATELET ERYTHROLEUKEMIA CELL PROSTAGLANDIN G/H SYNTHASE - CDNA CLONING, EXPRESSION, AND GENE CHROMOSOMAL ASSIGNMENT [J].

FUNK, CD ;

FUNK, LB ;

KENNEDY, ME ;

PONG, AS ;

FITZGERALD, GA .

FASEB JOURNAL, 1991, 5 (09) :2304-2312

[8] ANALYSIS OF CYTOKINE MESSENGER-RNA AND DNA - DETECTION AND QUANTITATION BY COMPETITIVE POLYMERASE CHAIN-REACTION [J].

GILLILAND, G ;

PERRIN, S ;

BLANCHARD, K ;

BUNN, HF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (07) :2725-2729

[9] SELECTIVE-INHIBITION OF INDUCIBLE CYCLOOXYGENASE-2 IN-VIVO IS ANTIINFLAMMATORY AND NONULCEROGENIC [J].

MASFERRER, JL ;

ZWEIFEL, BS ;

MANNING, PT ;

HAUSER, SD ;

LEAHY, KM ;

SMITH, WG ;

ISAKSON, PC ;

SEIBERT, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (08) :3228-3232

[10] CDNA CLONING AND FUNCTIONAL-ACTIVITY OF A GLUCOCORTICOID-REGULATED INFLAMMATORY CYCLOOXYGENASE [J].

OBANION, MK ;

WINN, VD ;

YOUNG, DA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (11) :4888-4892

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