IFN-γ:: Regulation of nitric oxide in the P-aeruginosa-infected cornea

被引：10

作者：

McClellan, SA ^{[1
]}

Lighvani, S ^{[1
]}

Hazlett, LD ^{[1
]}

机构：

[1] Wayne State Univ, Sch Med, Dept Anat & Cell Biol, Detroit, MI 48201 USA

来源：

OCULAR IMMUNOLOGY AND INFLAMMATION | 2006年 / 14卷 / 01期

基金：

美国国家卫生研究院;

关键词：

cytokines; inflammatory mediators; in-vivo animal model; infectious immunity bacteria;

D O I：

10.1080/09273940500545650

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Purpose: BALB/c mice are resistant to Pseudomonas aeruginosa (P. aeruginosa ) keratitis and bacterial killing/stasis requires nitric oxide (NO). NO regulation in the cornea is unknown and was tested in this model. Methods: Nitrite detection, IFN-gamma-knockout mice, TNF-alpha neutralization, ELISA, aminoguanidine (AG) treatment, MPO, and plate counts were done. Results: Evidence shows (i) without IFN-gamma, nitrite levels are elevated, (ii) neutralization of TNF-alpha does not change nitrite levels, and (iii) absence of IFN-gamma and reduced NO synergistically increases disease progress and upregulates pro-inflammatory cytokines, PMN number, and bacterial load. Conclusion: IFN-gamma regulates NO levels, and synergistic interaction between the two regulates disease outcome in resistant mice.

引用

页码：21 / 28

页数：8

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