Cosuppression of nonhomologous transgenes in Drosophila involves mutually related endogenous sequences

被引：63

作者：

Pal-Bhadra, M ^{[1
]}

Bhadra, U ^{[1
]}

Birchler, JA ^{[1
]}

机构：

[1] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA

来源：

CELL | 1999年 / 99卷 / 01期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/S0092-8674(00)80060-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cosuppression refers to the phenomenon in which silencing among dispersed homologous genes occurs. Here we demonstrate that two nonhomologous reciprocal fusion genes, white-Alcohol dehydrogenase (w-Adh) and Adh-w, exhibit cosuppression using the endogenous Adh sequence as an intermediary. Deletion of the endogenous Adh gene eliminates the interaction, while reintroduction of an 8.6 kb Adh fragment restores the silencing. Using truncated Adh constructs, a nontranscribed segment in the Adh regulatory region was found to be one of the sequences required for homology recognition. The silencing interaction is initiated during early development. The silenced transgenes are associated with the Polycomb group complex of chromatin proteins.

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页码：35 / 46

页数：12

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