Toxicity analysis of N- and C-terminus-deleted vegetative insecticidal protein from Bacillus thuringiensis

被引：79

作者：

Selvapandiyan, A

Arora, N

Rajagopal, R

Jalali, SK

Venkatesan, T

Singh, SP

Bhatnagar, RK

机构：

[1] Int Ctr Genet Engn & Biotechnol, New Delhi 110067, India

[2] Project Directorate Biol Control, Biotechnol Lab, Bangalore 560024, Karnataka, India

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2001年 / 67卷 / 12期

关键词：

D O I：

10.1128/AEM.67.12.5855-5858.2001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A vegetative insecticidal protein (VIP)-encoding gene from a local isolate of Bacillus thuringiensis has been cloned, sequenced, and expressed in Escherichia coli. The expressed protein shows insecticidal activity against several lepidopteran pests but is ineffective against Agrotis ipsilon. Comparison of the amino acid sequence with those of reported VIPs revealed a few differences. Analysis of insecticidal activity with N- and C-terminus deletion mutants suggests a differential mode of action of VIP against different pests.

引用

页码：5855 / 5858

页数：4

共 9 条

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