Construction of a combinatorial protein library displayed on yeast cell surface using DNA random priming method

被引：8

作者：

Zou, W ^{[1
]}

Ueda, M ^{[1
]}

Yamanaka, H ^{[1
]}

Tanaka, A ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Engn, Dept Synthet Chem & Biol Chem, Lab Appl Biol Chem,Sakyo Ku, Kyoto 6068501, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2001年 / 92卷 / 04期

关键词：

combinatorial random protein library; DNA random priming; yeast cell surface engineering; surface display;

D O I：

10.1263/jbb.92.393

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The random DNA fragments, generated from mRNAs of Saccharomyces cerevisiae using a novel "DNA random priming method", were inserted into a surface display vector to construct a combinatorial random protein library. Its surface display was confirmed by immunofluorescence labeling of the RGS (His)(6) reporter inserted, and its potential was also evaluated.

引用

页码：393 / 396

页数：4

共 20 条

[1]

[Anonymous], 1994, METHODS YEAST GENETI

[2] Yeast surface display for screening combinatorial polypeptide libraries [J].