Protein-mediated particle assembly

被引:46
作者
Brown, S [1 ]
机构
[1] Univ Copenhagen, Dept Mol Cell Biol, DK-1353 Copenhagen K, Denmark
关键词
D O I
10.1021/nl0155173
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
This report describes a bifunctional protein able to associate dissimilar materials. One domain of the protein adheres to metallic gold. The other domain contains a substrate for enzymatic biotin transfer. The protein is able to assemble micron-diameter gold and streptavidin-coated polystyrene spheres in a programmed manner. Both protein domains function independently and therefore can be fused to proteins displaying a variety of binding and assembly traits.
引用
收藏
页码:391 / 394
页数:4
相关论文
共 20 条
[1]   A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation [J].
Beckett, D ;
Kovaleva, E ;
Schatz, PJ .
PROTEIN SCIENCE, 1999, 8 (04) :921-929
[2]   Cell-surface display of a Pseudomonas aeruginosa strain K pilin peptide within the paracrystalline S-layer of Caulobacter crescentus [J].
Bingle, WH ;
Nomellini, JF ;
Smit, J .
MOLECULAR MICROBIOLOGY, 1997, 26 (02) :277-288
[3]   Metal-recognition by repeating polypeptides [J].
Brown, S .
NATURE BIOTECHNOLOGY, 1997, 15 (03) :269-272
[4]   A genetic analysis of crystal growth [J].
Brown, S ;
Sarikaya, M ;
Johnson, E .
JOURNAL OF MOLECULAR BIOLOGY, 2000, 299 (03) :725-735
[5]  
Cronan JE, 2000, METHOD ENZYMOL, V326, P440
[6]  
Cull MG, 2000, METHOD ENZYMOL, V326, P430
[7]   Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein [J].
Howorka, S ;
Sára, M ;
Wang, YJ ;
Kuen, B ;
Sleytr, UB ;
Lubitz, W ;
Bayley, H .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (48) :37876-37886
[8]   PROTEIN ENGINEERING OF ANTIBODY-BINDING SITES - RECOVERY OF SPECIFIC ACTIVITY IN AN ANTI-DIGOXIN SINGLE-CHAIN FV ANALOG PRODUCED IN ESCHERICHIA-COLI [J].
HUSTON, JS ;
LEVINSON, D ;
MUDGETTHUNTER, M ;
TAI, MS ;
NOVOTNY, J ;
MARGOLIES, MN ;
RIDGE, RJ ;
BRUCCOLERI, RE ;
HABER, E ;
CREA, R ;
OPPERMANN, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (16) :5879-5883
[9]   Biotinylation in vivo as a sensitive indicator of protein secretion and membrane protein insertion [J].
Jander, G ;
Cronan, JE ;
Beckwith, J .
JOURNAL OF BACTERIOLOGY, 1996, 178 (11) :3049-3058
[10]   THE MECHANISM OF BIOTIN-DEPENDENT ENZYMES [J].
KNOWLES, JR .
ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :195-221