Spectrophotometric measurement of carotenes, xanthophylls, and chlorophylls in extracts from plant seeds

被引：40

作者：

Bulda, O. V. ^{[1
]}

Rassadina, V. V. ^{[2
]}

Alekseichuk, H. N. ^{[1
]}

Laman, N. A. ^{[1
]}

机构：

[1] Natl Acad Sci Belarus, Kuprevich Inst Expt Bot, Minsk 220072, BELARUS

[2] Natl Acad Sci Belarus, Inst Biophys & Cell Engn, Minsk 220072, BELARUS

来源：

RUSSIAN JOURNAL OF PLANT PHYSIOLOGY | 2008年 / 55卷 / 04期

关键词：

plants; seeds; carotene; xanthophylls; chlorophylls a and b; spectrophotometry; equations for calculation of pigment content;

D O I：

10.1134/S1021443708040171

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The method of spectrophotometric measurement of carotenoid and chlorophyll content in extracts from plant seeds was modified. The pigments were extracted with a mixture of petroleum ether (PE) and tetrahydrofuran (THF) (PE to THF ratio 4: 1). Equations for calculations of beta-carotene, lutein, chlorophyll a and b content in PE: THF mixture were obtained using specific absorption coefficients of solutions of particular pigments. It was shown that subtraction of chlorophyll contribution from the absorption spectrum of seed extracts yields spectra, which could be used in some cases for calculation of carotene and xanthophyll contents.

引用

页码：544 / 551

页数：8

共 33 条

[1]

AKULOVICH NK, 1983, FORMATION STATE PHOT

[2]

BERSHTEIN IY, 1986, SPEKTROFOTOMETRICHES

[3]

DAVIES BH, 1965, CHEM BIOCH PLANT PIG, P183

[4] Antioxidants, free radicals, storage proteins, and proteolytic activities in wheat (Triticum durum) seeds during accelerated aging [J].

Galleschi, L ;

Capocchi, A ;

Ghiringhelli, S ;

Saviozzi, F ;

Calucci, L ;

Pinzino, C ;

Zandomeneghi, M .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (19) :5450-5457

[5]

Goodwin T.W., 1980, BIOCH CAROTENOIDS, V1

[6] Carotenoids as membrane stabilizers in chloroplasts [J].

Havaux, M .

TRENDS IN PLANT SCIENCE, 1998, 3 (04) :147-151

[7] Evaluation of analytical methods for carotenoid extraction from biofortified maize (Zea mays sp.) [J].

Howe, Julie A. ;

Tanumihardjo, Sherry A. .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (21) :7992-7997

[8] Carotenoid accumulation and function in seeds and non-green tissues [J].

Howitt, CA ;

Pogson, BJ .

PLANT CELL AND ENVIRONMENT, 2006, 29 (03) :435-445

[9] Chlorophyll fluorescence of Brassica oleracea seeds as a non-destructive marker for seed maturity and seed performance [J].

Jalink, H ;

van der Schoor, R ;

Frandas, A ;

van Pijlen, JG ;

Bino, RJ .

SEED SCIENCE RESEARCH, 1998, 8 (04) :437-443

[10]

Kaiser R, 2001, CHEM UNSERER ZEIT, V35, P8, DOI 10.1002/1521-3781(200101)35:1<8::AID-CIUZ8>3.0.CO

← 1 2 3 4 →