Development of ITS sequence-based markers to distinguish Berberis aristata DC. from B. lycium Royle and B. asiatica Roxb.

被引：17

作者：

Balasubramani, Subramani Paranthaman ^{[1
]}

Goraya, Gurinder Singh ^{[2
]}

Venkatasubramanian, Padma ^{[1
]}

机构：

[1] Fdn Revitalizat Local Hlth Tradit, Inst Ayurveda & Integrat Med I AIM, Ctr Pharmacognosy Pharmaceut & Pharmacol, Bangalore 560106, Karnataka, India

[2] Indian Forest Serv, Mandi 174402, Himachal Prades, India

来源：

3 BIOTECH | 2011年 / 1卷 / 01期

关键词：

Daruharidra; Berberis spp; Quality control; Internal transcribed spacer (ITS); DNA marker; AUTHENTICATION;

D O I：

10.1007/s13205-010-0001-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

The stems of Berberis aristata DC. (Berberidaceae) are used in the South Asian traditional medicine as a key ingredient in formulations for eye care, skin diseases, jaundice, rheumatism and also in diabetes. B. lycium Royle and B. asiatica Roxb. are traded as equivalents of B. aristata. Conventional macro-morphology and microscopic examination does not aid in critically distinguishing the three species. DNA markers were developed by amplifying and sequencing the complete internal transcribed spacer region (ITS1, 5.8S rRNA and ITS2) from the genomic DNA, using universal primers. The markers developed are efficient and reliable in authenticating B. aristata, B. asiatica and B. lycium. These are useful as molecular pharmacognostic tool in quality control of raw drugs.

引用

页码：11 / 19

页数：9

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