Enhanced expression of an antibody-targeted plasminogen activator in Escherichia coli by fusion to decorsin

被引：3

作者：

Yu, M ^{[1
]}

Yu, MM ^{[1
]}

Ru, BG ^{[1
]}

机构：

[1] Peking Univ, Coll Life Sci, Natl Key Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 09期

关键词：

antibody-targeted plasminogen activator; decorsin; platelet binding; thrombolysis;

D O I：

10.1023/B:BILE.0000024095.83712.5e

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To improve the thrombolytic specificity of plasminogen activators, an antibody-targeted plasminogen activator was constructed consisting of a single-chain variable fragment of a monoclonal antibody SZ-51 raised specifically against human P-selectins on activated platelets and a low molecular weight single-chain urokinase. After fusion to the 3' end of the gene coding for decorsin, originally isolated from the leech Macrobdella decora, expression of the antibody-targeted plasminogen activator gene in E. coli strain Rosetta (DE3) pLysS was greatly enhanced.

引用

页码：717 / 721

页数：5

共 11 条

[1]

Becker Richard C., 1999, American Journal of Cardiology, V83, p3E

[2]

Collen D, 1997, THROMB HAEMOSTASIS, V78, P742

[3] TOWARDS IMPROVED THROMBOLYTIC THERAPY [J].